通过一种一体化策略在拟南芥中进行基因靶向，该策略使用翻译增强子来辅助Cas9表达。 - Suppr

Gene targeting in Arabidopsis via an all-in-one strategy that uses a translational enhancer to aid Cas9 expression.

作者信息

Peng Fangnan, Zhang Wenxin, Zeng Wenjie, Zhu Jian-Kang, Miki Daisuke

机构信息

Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.

University of Chinese Academy of Sciences, Beijing, China.

出版信息

Plant Biotechnol J. 2020 Apr;18(4):892-894. doi: 10.1111/pbi.13265. Epub 2019 Oct 7.

DOI:10.1111/pbi.13265

PMID:31553828

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7061861/

Abstract

摘要

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Gene targeting in Arabidopsis via an all-in-one strategy that uses a translational enhancer to aid Cas9 expression.通过一种一体化策略在拟南芥中进行基因靶向，该策略使用翻译增强子来辅助Cas9表达。

Plant Biotechnol J. 2020 Apr;18(4):892-894. doi: 10.1111/pbi.13265. Epub 2019 Oct 7.

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Plant Methods. 2018 Dec 18;14:111. doi: 10.1186/s13007-018-0382-8. eCollection 2018.

Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato.利用翻译增强子 dMac3 和多个向导 RNA 在马铃薯中建立具有更高诱变频率的改良 CRISPR/Cas9 系统。

Sci Rep. 2018 Sep 13;8(1):13753. doi: 10.1038/s41598-018-32049-2.

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Nat Commun. 2018 May 17;9(1):1967. doi: 10.1038/s41467-018-04416-0.

Efficient in planta gene targeting in Arabidopsis using egg cell-specific expression of the Cas9 nuclease of Staphylococcus aureus.利用金黄色葡萄球菌 Cas9 核酸酶在拟南芥卵细胞中特异性表达实现高效的体内基因靶向。

Plant J. 2018 May;94(4):735-746. doi: 10.1111/tpj.13893. Epub 2018 Apr 17.

Origins of Programmable Nucleases for Genome Engineering.用于基因组工程的可编程核酸酶的起源

J Mol Biol. 2016 Feb 27;428(5 Pt B):963-89. doi: 10.1016/j.jmb.2015.10.014. Epub 2015 Oct 23.

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In planta gene targeting.在植物体内进行基因靶向。

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Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells.通过基因靶向在小鼠胚胎衍生干细胞中进行定点诱变。

Cell. 1987 Nov 6;51(3):503-12. doi: 10.1016/0092-8674(87)90646-5.

A translational enhancer derived from tobacco mosaic virus is functionally equivalent to a Shine-Dalgarno sequence.一种源自烟草花叶病毒的翻译增强子在功能上等同于SD序列。

Proc Natl Acad Sci U S A. 1989 Jan;86(1):129-32. doi: 10.1073/pnas.86.1.129.

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Gene targeting in Arabidopsis via an all-in-one strategy that uses a translational enhancer to aid Cas9 expression.

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