一种源自烟草花叶病毒的翻译增强子在功能上等同于SD序列。
A translational enhancer derived from tobacco mosaic virus is functionally equivalent to a Shine-Dalgarno sequence.
作者信息
Gallie D R, Kado C I
机构信息
Department of Plant Pathology, University of California Davis, 95616.
出版信息
Proc Natl Acad Sci U S A. 1989 Jan;86(1):129-32. doi: 10.1073/pnas.86.1.129.
Abstract
When present at the 5' end of mRNAs, the untranslated leader sequence (omega) of tobacco mosaic virus RNA significantly enhances translation in eukaryotes and prokaryotes. We have tested a deletion derivative of the omega sequence, omega delta 3, for its enhancing ability on gene constructs in which the ribosomal binding site was either present or deleted, in several Gram-negative bacterial species including Escherichia coli, Agrobacterium tumefaciens, Xanthomonas campestris pv. vitians, Erwinia amylovora, and Salmonella typhimurium. In vivo production of chloramphenicol acetyltransferase from a gene construct lacking its native ribosomal binding site was enhanced 40- to 120-fold by the presence of omega delta 3. Similar levels of enhancement (30- to 240-fold) were observed when the gene encoding beta-glucuronidase was tested. With a chloramphenicol acetyltransferase construct containing a ribosomal binding site, enhancement was markedly less, between 1- and 3.8-fold. Omega delta 3 appeared to enhance translation independent of its position upstream of the AUG codon used for initiation.
摘要
当烟草花叶病毒RNA的非翻译前导序列（ω）出现在mRNA的5'端时，它能显著增强真核生物和原核生物中的翻译。我们已经在包括大肠杆菌、根癌农杆菌、野油菜黄单胞菌致病变种、梨火疫病菌和鼠伤寒沙门氏菌在内的几种革兰氏阴性细菌中，测试了ω序列的缺失衍生物ωδ3对核糖体结合位点存在或缺失的基因构建体的增强能力。对于一个缺乏天然核糖体结合位点的基因构建体，ωδ3的存在使其氯霉素乙酰转移酶的体内产量提高了40至120倍。当测试编码β-葡萄糖醛酸酶的基因时，也观察到了类似水平的增强（30至240倍）。对于一个含有核糖体结合位点的氯霉素乙酰转移酶构建体，增强作用明显较小，仅为1至3.8倍。ωδ3似乎能独立于其在用于起始的AUG密码子上游的位置增强翻译。
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