兔补体C3、B因子和H因子的分离及其与人类相应因子特性的比较。

Isolation of rabbit C3, Factor B, and Factor H and comparison of their properties with those of the human analog.

作者信息

Horstmann R D, Müller-Eberhard H J

出版信息

J Immunol. 1985 Feb;134(2):1094-100.

Abstract

The rabbit complement components C3, Factor B, and Factor H were isolated and characterized and were compared to the corresponding proteins of human serum. Chromatographic behavior, chemical properties, and functional interactions show great similarities between the components in both species. By SDS polyacrylamide gel electrophoresis, the m.w. were estimated to be 195,000 for C3, 86,000 for Factor B, and 155,000 for Factor H. The amino acid compositions of the rabbit proteins resembled those of the human analog. The total carbohydrate content of rabbit C3 and Factor H was approximately one-half that of the human proteins. In addition, a qualitative difference in the carbohydrate moieties of the C3 proteins was observed. The serum concentration of the rabbit proteins was markedly lower than that of the human proteins. The rabbit C3b,Bb enzyme resembled the human analog with respect to half-life, control by Factor H, and stabilization by nickel ions.

摘要

兔补体成分C3、B因子和H因子被分离并进行了特性鉴定，并与人类血清中的相应蛋白质进行了比较。色谱行为、化学性质和功能相互作用表明，这两个物种的成分之间存在很大的相似性。通过SDS聚丙烯酰胺凝胶电泳，估计C3的分子量为195,000，B因子为86,000，H因子为155,000。兔蛋白的氨基酸组成与人类类似物相似。兔C3和H因子的总碳水化合物含量约为人类蛋白质的一半。此外，观察到C3蛋白碳水化合物部分的质量差异。兔蛋白的血清浓度明显低于人类蛋白。兔C3b,Bb酶在半衰期、H因子控制和镍离子稳定方面与人类类似物相似。