Reproductive & Genetic Center, National Research Institute for Family Planning, Beijing 100081, PR China.
Key Laboratory of Molecular Developmental Biology, Institute of Genetics & Developmental Biology, Chinese Academy of Sciences, Beijing 100080, PR China.
Regen Med. 2019 Oct;14(10):915-923. doi: 10.2217/rme-2018-0134. Epub 2019 Sep 26.
The major symptom of many closed spinal dysraphism patients is that the laminas or arches of vertebra are not fused well. To date, the bone repair of spina bifida for young children is a significant challenge in clinical practice. Bovine bone collagen particle (BBCP) scaffolds combined with human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were implanted in the defect area. X-ray analysis was performed after 3 months. Tissues were harvested for gross observation, and histological and immunohistochemical staining. The BBCP supported hUC-MSCs adhesion and growth. Implanted BBCP combined with hUC-MSCs also promoted bone regeneration in the vertebral lamina and arch defect area. This method represents a new strategy for vertebral lamina and arch reconstruction in children.
许多闭合性脊柱脊膜膨出患者的主要症状是椎板或椎弓未融合好。迄今为止，儿童的脊裂骨修复在临床实践中是一个重大挑战。将牛骨胶原颗粒（BBCP）支架与人类脐带间充质干细胞（hUC-MSCs）联合植入缺损区域。3 个月后进行 X 射线分析。采集组织进行大体观察、组织学和免疫组织化学染色。BBCP 支持 hUC-MSCs 的黏附和生长。植入的 BBCP 与 hUC-MSCs 联合也促进了椎板和椎弓缺损区域的骨再生。该方法为儿童椎板和椎弓重建提供了一种新策略。
