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兔骨胶原颗粒与人脐带间充质干细胞联合修复牙槽裂骨缺损。

Repair of alveolar cleft bone defects by bone collagen particles combined with human umbilical cord mesenchymal stem cells in rabbit.

机构信息

Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, 10081, China.

Graduate Schools, Peking Union Medical College, Beijing, 100730, China.

出版信息

Biomed Eng Online. 2020 Aug 3;19(1):62. doi: 10.1186/s12938-020-00800-4.

DOI:10.1186/s12938-020-00800-4
PMID:32746926
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7397686/
Abstract

BACKGROUND

Alveolar cleft is a type of cleft lip and palate that seriously affects the physical and mental health of patients. In this study, a model of the alveolar cleft phenotype was established in rabbits to evaluate the effect of bone collagen particles combined with human umbilical cord mesenchymal stem cells (HUC-MSCs) on the repair of alveolar cleft bone defects.

METHODS

A model of alveolar clefts in rabbits was established by removing the incisors on the left side of the upper jaw bone collagen particles combined with HUC-MSCs that were then implanted in the defect area. Blood biochemical analysis was performed 3 months after surgery. Skull tissues were harvested for gross observation, and micro-focus computerised tomography (micro-CT) analysis. Tissues were harvested for histological and immunohistochemical staining. The experiments were repeated 6 months after surgery.

RESULTS

Bone collagen particles and HUC-MSCs showed good biocompatibility. Bone collagen particles combined with HUC-MSCs were markedly better at inducing bone repair and regeneration than bone collagen particles alone.

CONCLUSIONS

Combining HUC-MSCs with bone collagen particles provides a simple, rapid and suitable method to fill a bone defect site and treat of alveolar cleft bone defects.

摘要

背景

牙槽裂是一种唇腭裂，严重影响患者的身心健康。本研究通过建立兔牙槽裂模型，评价骨胶原颗粒联合人脐带间充质干细胞（HUC-MSCs）对牙槽裂骨缺损修复的影响。

方法

通过在上颌骨左侧切牙部位去除牙槽骨，建立兔牙槽裂模型，将骨胶原颗粒联合 HUC-MSCs 植入缺损区。术后 3 个月进行血液生化分析。术后 6 个月取颅骨组织进行大体观察和微焦点计算机断层扫描（micro-CT）分析，取组织行组织学和免疫组织化学染色。实验重复 6 个月。

结果

骨胶原颗粒与 HUC-MSCs 具有良好的生物相容性。骨胶原颗粒联合 HUC-MSCs 明显优于单纯骨胶原颗粒，更能诱导骨修复和再生。

结论

HUC-MSCs 联合骨胶原颗粒为填充骨缺损部位和治疗牙槽裂骨缺损提供了一种简单、快速、适宜的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f86/7397686/03a4af035e68/12938_2020_800_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f86/7397686/6438dd1e3b26/12938_2020_800_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f86/7397686/7fa85a6faf39/12938_2020_800_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f86/7397686/03a4af035e68/12938_2020_800_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f86/7397686/6438dd1e3b26/12938_2020_800_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f86/7397686/6c26d9d08a45/12938_2020_800_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f86/7397686/ccdf6d946855/12938_2020_800_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f86/7397686/8939b536c2b7/12938_2020_800_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f86/7397686/d524c52f97a2/12938_2020_800_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f86/7397686/2f5dd4aa55c9/12938_2020_800_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f86/7397686/06f4968d985d/12938_2020_800_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f86/7397686/59697e171758/12938_2020_800_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f86/7397686/7fa85a6faf39/12938_2020_800_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f86/7397686/03a4af035e68/12938_2020_800_Fig10_HTML.jpg

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本文引用的文献

1
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Int J Pediatr Otorhinolaryngol. 2019 Sep;124:164-172. doi: 10.1016/j.ijporl.2019.05.002. Epub 2019 May 31.
2
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Clin Orthop Surg. 2018 Sep;10(3):271-278. doi: 10.4055/cios.2018.10.3.271. Epub 2018 Aug 22.
3
Novel antibacterial biodegradable Fe-Mn-Ag alloys produced by mechanical alloying.机械合金化制备新型抗菌可生物降解 Fe-Mn-Ag 合金。
CA1 通过体外激活 BMP 信号通路调节牙滤泡干细胞的成骨分化。
Tissue Eng Regen Med. 2024 Aug;21(6):855-865. doi: 10.1007/s13770-024-00642-4. Epub 2024 Apr 23.
4
Stem Cell-Based Tissue Engineering for Cleft Defects: Systematic Review and Meta-Analysis.基于干细胞的组织工程学治疗裂隙缺陷：系统评价和荟萃分析。
Cleft Palate Craniofac J. 2024 Sep;61(9):1439-1460. doi: 10.1177/10556656231175278. Epub 2023 May 18.
5
Research Progress on the Osteogenesis-Related Regulatory Mechanisms of Human Umbilical Cord Mesenchymal Stem Cells.人脐带间充质干细胞成骨相关调控机制的研究进展
Stem Cell Rev Rep. 2023 Jul;19(5):1252-1267. doi: 10.1007/s12015-023-10521-5. Epub 2023 Mar 14.
6
A Critical Review of Additive Manufacturing Techniques and Associated Biomaterials Used in Bone Tissue Engineering.骨组织工程中增材制造技术及相关生物材料的批判性综述。
Polymers (Basel). 2022 May 23;14(10):2117. doi: 10.3390/polym14102117.
7
Use of stem cells in bone regeneration in cleft palate patients: review and recommendations.干细胞在腭裂患者骨再生中的应用：综述与建议。
J Korean Assoc Oral Maxillofac Surg. 2022 Apr 30;48(2):71-78. doi: 10.5125/jkaoms.2022.48.2.71.
8
Human Umbilical Cord Mesenchymal Stem Cells: Current Literature and Role in Periodontal Regeneration.人脐带间充质干细胞：当前文献及在牙周再生中的作用。
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9
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Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2021 Mar 15;35(3):295-302. doi: 10.7507/1002-1892.202009070.
Mater Sci Eng C Mater Biol Appl. 2018 Jul 1;88:88-94. doi: 10.1016/j.msec.2018.03.005. Epub 2018 Mar 11.
4
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5
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7
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9
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