Forni M, Hofman F M, Parker J W, Lukes R J, Taylor C R
Cancer. 1985 Feb 15;55(4):728-37. doi: 10.1002/1097-0142(19850215)55:4<728::aid-cncr2820550409>3.0.co;2-s.
Immunohistologic techniques utilizing monoclonal antibodies have made possible the identification of leukocytes by their phenotypic characteristics rather than by morphologic features alone. A panel of antibodies for T-lymphocyte, B-lymphocyte, and monocyte/histiocyte markers has been applied to frozen sections of nodular sclerosing and mixed cellularity Hodgkin's disease to identify more precisely the various cell types present in tissues involved in Hodgkin's disease. The majority of lymphocytes expressed detectable T-cell phenotypic markers, with a predominance of the "helper" phenotype (Leu 3/OKT4) in most cases. Lymphocytes reacting with anti-B-cell antibodies were also demonstrated; their distribution is described here and has not previously been reported. The anti-B- and anti-T-cell antibodies used in this study failed to give positive reactivity with Reed-Sternberg cells. However, one of the anti-monocyte antibodies (Leu-M1) reacted with diagnostic Reed-Sternberg cells in some cases. The patterns of staining observed varied widely within the two histologic types of Hodgkin's disease, leading to a conclusion that this disease may be more heterogeneous than is currently suspected.
利用单克隆抗体的免疫组织学技术使得通过白细胞的表型特征而非仅通过形态学特征来识别白细胞成为可能。一组针对T淋巴细胞、B淋巴细胞和单核细胞/组织细胞标志物的抗体已应用于结节硬化型和混合细胞型霍奇金病的冰冻切片，以更精确地识别霍奇金病受累组织中存在的各种细胞类型。大多数淋巴细胞表达可检测到的T细胞表型标志物，在大多数情况下以“辅助性”表型（Leu 3/OKT4）为主。与抗B细胞抗体反应的淋巴细胞也得到了证实；本文描述了它们的分布情况，此前尚未有报道。本研究中使用的抗B细胞和抗T细胞抗体与里德-斯腾伯格细胞未产生阳性反应。然而，一种抗单核细胞抗体（Leu-M1）在某些情况下与诊断性里德-斯腾伯格细胞发生反应。在霍奇金病的两种组织学类型中观察到的染色模式差异很大，由此得出结论，这种疾病可能比目前所怀疑的更加异质性。
