College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China.
Food Funct. 2019 Oct 16;10(10):6666-6674. doi: 10.1039/c9fo01273g.
Animal protein intake appears to exert beneficial effects on bone health. Here, animal protein hydrolysates (APHs) originated from casein, whey protein isolate, egg white, myofibrillar protein, sarcoplasmic protein and gelatin were shown to prevent calcium phosphate (CaP) precipitation by increasing the surface charge and slowing the crystal growth of CaP particles. Dynamic light scattering, transmission electron microscopy and energy-dispersive X-ray analysis revealed the APH-mediated formation of irregularly shaped secondary nanoparticles aggregated from rather small amorphous CaP nanoclusters. APHs effectively counteracted the detrimental effect of the low calcium-to-phosphorus ratio on calcium transportation across ligated murine ileum, and by treating with NaN3, amiloride and low temperature, macropinocytosis was found to involve the intestinal uptake of CaP nanoparticles. APHs of meat origin had weaker potential to increase CaP solubility and ileal calcium transportation than those of dairy and egg origins. Overall, our study reports a novel mechanism for animal protein intake to promote intestinal calcium absorption.
动物蛋白摄入似乎对骨骼健康有益。在这里，酪蛋白、乳清蛋白分离物、蛋清、肌原纤维蛋白、肌浆蛋白和明胶来源的动物蛋白水解物 (APHs) 被证明可以通过增加表面电荷和减缓 CaP 颗粒的晶体生长来防止磷酸钙 (CaP) 沉淀。动态光散射、透射电子显微镜和能量色散 X 射线分析揭示了 APH 介导的形成不规则形状的二次纳米颗粒，这些颗粒由相当小的无定形 CaP 纳米簇聚集而成。APHs 有效地抵消了低钙磷比对结扎的小鼠回肠钙转运的不利影响，并且通过用NaN3、阿米洛利和低温处理，发现巨胞饮作用涉及 CaP 纳米颗粒的肠道摄取。与乳源和蛋源 APHs 相比，肉源 APHs 增加 CaP 溶解度和回肠钙转运的潜力较弱。总的来说，我们的研究报告了一种动物蛋白摄入促进肠道钙吸收的新机制。
