用于心脏递送微小RNA的仿生带负电磷酸钙纳米载体
Bioinspired negatively charged calcium phosphate nanocarriers for cardiac delivery of MicroRNAs.
作者信息
Di Mauro Vittoria, Iafisco Michele, Salvarani Nicolò, Vacchiano Marco, Carullo Pierluigi, Ramírez-Rodríguez Gloria Belén, Patrício Tatiana, Tampieri Anna, Miragoli Michele, Catalucci Daniele
机构信息
National Research Council (CNR), Institute of Genetics & Biomedical Research, Milan Unit, Milan 20138, Italy.
Humanitas Clinical & Research Center, Rozzano (MI) 20089, Italy.
出版信息
Nanomedicine (Lond). 2016 Apr;11(8):891-906. doi: 10.2217/nnm.16.26. Epub 2016 Mar 16.
AIM
To develop biocompatible and bioresorbable negatively charged calcium phosphate nanoparticles (CaP-NPs) as an innovative therapeutic system for the delivery of bioactive molecules to the heart.
MATERIALS & METHODS: CaP-NPs were synthesized via a straightforward one-pot biomineralization-inspired protocol employing citrate as a stabilizing agent and regulator of crystal growth. CaP-NPs were administered to cardiac cells in vitro and effects of treatments were assessed. CaP-NPs were administered in vivo and delivery of microRNAs was evaluated.
RESULTS
CaP-NPs efficiently internalized into cardiomyocytes without promoting toxicity or interfering with any functional properties. CaP-NPs successfully encapsulated synthetic microRNAs, which were efficiently delivered into cardiac cells in vitro and in vivo.
CONCLUSION
CaP-NPs are a safe and efficient drug-delivery system for potential therapeutic treatments of polarized cells such as cardiomyocytes.
目的
开发具有生物相容性和生物可吸收性的带负电荷的磷酸钙纳米颗粒（CaP-NPs），作为一种将生物活性分子输送到心脏的创新治疗系统。
材料与方法
通过一种简单的一锅法生物矿化启发方案合成CaP-NPs，该方案使用柠檬酸盐作为稳定剂和晶体生长调节剂。将CaP-NPs体外施用于心脏细胞，并评估治疗效果。将CaP-NPs体内给药，并评估微小RNA的递送情况。
结果
CaP-NPs能有效内化到心肌细胞中，而不会促进毒性或干扰任何功能特性。CaP-NPs成功包裹了合成的微小RNA，这些微小RNA在体外和体内均能有效地递送至心脏细胞。
结论
CaP-NPs是一种安全有效的药物递送系统，可用于对极化细胞如心肌细胞进行潜在的治疗。
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