Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada; Radiation Oncology, Sunnybrook Health Sciences Centre, Toronto, ON, Canada.
Radiation Oncology, Sunnybrook Health Sciences Centre, Toronto, ON, Canada.
Neuroimage. 2020 Jan 1;204:116202. doi: 10.1016/j.neuroimage.2019.116202. Epub 2019 Sep 23.
Lactate is now recognized as an important intermediate in brain metabolism, but its role is still under investigation. In this work we mapped the distribution of lactate and bicarbonate produced from intravenously injected C-pyruvate over the whole brain using a new imaging method, hyperpolarized C MRI (N = 14, ages 23 to 77). Segmenting the C-lactate images into brain atlas regions revealed a pattern of lactate that was preserved across individuals. Higher lactate signal was observed in cortical grey matter compared to white matter and was highest in the precuneus, cuneus and lingual gyrus. Bicarbonate signal, indicating flux of [1-C]pyruvate into the TCA cycle, also displayed consistent spatial distribution. One-way ANOVA to test for significant differences in lactate among atlas regions gave F = 87.6 and p < 10. This report of a "lactate topography" in the human brain and its consistent pattern is evidence of region-specific lactate biology that is preserved across individuals.
乳酸现在被认为是大脑代谢中的一个重要中间产物，但它的作用仍在研究中。在这项工作中，我们使用一种新的成像方法——超极化 C MRI 来绘制整个大脑中静脉注射 C-丙酮酸产生的乳酸和碳酸氢盐的分布（N=14，年龄 23 至 77 岁）。将 C-乳酸图像分割成大脑图谱区域，揭示了一种在个体之间保持不变的乳酸模式。与白质相比，皮质灰质中的乳酸信号更高，在楔前叶、楔叶和舌回中最高。表明 [1-C]丙酮酸流入三羧酸循环通量的碳酸氢盐信号也显示出一致的空间分布。单因素方差分析（one-way ANOVA）检验图谱区域之间乳酸的显著差异，得到 F=87.6，p<10。本报告中人类大脑中存在“乳酸地形图”及其一致模式的证据表明，乳酸生物学在个体之间是具有区域特异性的。
