Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.
Int J Mol Sci. 2019 Sep 26;20(19):4787. doi: 10.3390/ijms20194787.
The application of purified enzymes as well as whole-cell biocatalysts in synthetic organic chemistry is becoming more and more popular, and both academia and industry are keen on finding and developing novel enzymes capable of performing otherwise impossible or challenging reactions. The diverse genus offers a multitude of promising enzymes, which therefore makes it one of the key bacterial hosts in many areas of research. This review focused on the broad utilization potential of the genus in organic chemistry, thereby particularly highlighting the specific enzyme classes exploited and the reactions they catalyze. Additionally, close attention was paid to the substrate scope that each enzyme class covers. Overall, a comprehensive overview of the applicability of the genus is provided, which puts this versatile microorganism in the spotlight of further research.
在合成有机化学中,纯化酶以及全细胞生物催化剂的应用变得越来越流行,学术界和工业界都热衷于寻找和开发能够进行原本不可能或具有挑战性的反应的新型酶。属提供了多种有前途的酶,因此使其成为许多研究领域的关键细菌宿主之一。本综述重点介绍了属在有机化学中的广泛应用潜力,特别强调了所利用的特定酶类及其催化的反应。此外,还密切关注了每个酶类涵盖的底物范围。总的来说,提供了属的适用性的全面概述,使这个多功能的微生物成为进一步研究的焦点。