Thomas D D, Baseman J B, Alderete J F
J Exp Med. 1985 Mar 1;161(3):514-25. doi: 10.1084/jem.161.3.514.
The specificity of the interaction between Treponema pallidum and fibronectin was demonstrated. Treatment of host cells with only antifibronectin sera and not anticollagen or antilaminin sera, inhibited treponemal cytadsorption. Incubation of fibronectin-coated coverslips with monoclonal antibody to the cell-binding domain of fibronectin reduced treponemal attachment to the same extent as antifibronectin serum. Both iodinated fibronectin and iodinated cell-binding domain bound to T. pallidum in a saturable manner. Specificity of the T. pallidum association with the cell-binding domain was the most effective inhibitor of the binding of either radioiodinated cell-binding domain or fibronectin to T. pallidum. Scatchard analysis gave Kd on the order of 10(-7) M for both cell-binding domain and fibronectin binding to T. pallidum, consistent with the high affinity interaction of these organisms with host cell surfaces. Finally, the same level of attachment of treponemes was achieved on coverslips coated with cell-binding domain as that observed for organisms incubated with fibronectin, indicating that the cell-binding domain polypeptide is functionally identical to fibronectin in mediating T. pallidum adherence.
梅毒螺旋体与纤连蛋白之间相互作用的特异性得到了证实。仅用抗纤连蛋白血清而非抗胶原蛋白或抗层粘连蛋白血清处理宿主细胞，可抑制梅毒螺旋体的细胞吸附。用针对纤连蛋白细胞结合结构域的单克隆抗体孵育纤连蛋白包被的盖玻片，其对梅毒螺旋体附着的抑制程度与抗纤连蛋白血清相同。碘化纤连蛋白和碘化细胞结合结构域均以可饱和的方式与梅毒螺旋体结合。梅毒螺旋体与细胞结合结构域结合的特异性是放射性碘化细胞结合结构域或纤连蛋白与梅毒螺旋体结合的最有效抑制剂。Scatchard分析得出细胞结合结构域和纤连蛋白与梅毒螺旋体结合的解离常数（Kd）约为10^(-7) M，这与这些生物体与宿主细胞表面的高亲和力相互作用一致。最后，在细胞结合结构域包被的盖玻片上观察到的梅毒螺旋体附着水平与用纤连蛋白孵育的生物体所观察到的相同，表明细胞结合结构域多肽在介导梅毒螺旋体黏附中的功能与纤连蛋白相同。
