Alderete J F, Baseman J B
Br J Vener Dis. 1981 Oct;57(5):302-8. doi: 10.1136/sti.57.5.302.
Sensitive and highly specific radioimmunoprecipitation and gel electrophoreses-fluorography were used to monitor the serum IgG response of experimentally infected rabbits against key protein antigens of Treponema pallidum. The sera from six rabbits challenged intradermally (ID) and six intratesticularly (IT) with T pallidum were examined over a period of four months. A specific antibody response to most treponemal surface proteins (including those ligands implicated as mediating host-cell-surface adherence) developed by about day 20 after inoculation. Maximum antibody concentrations against most of these immunogens were detected by day 50 in all animals irrespective of the route of inoculation. Differences as well as similarities in the IgG response to infection were observed among selected rabbits. Furthermore, the antibody reactivity of each animal was comparable to that of human sera from patients with secondary syphilis. Antibody directed against treponemal outer envelope proteins was detected by preferential agglutination of washed radiolabelled organisms with protein A-bearing Staphylococcus aureus in the presence of syphilitic rabbit sera.
采用灵敏且高度特异的放射免疫沉淀及凝胶电泳 - 荧光自显影技术，监测实验感染兔血清中针对梅毒螺旋体关键蛋白抗原的IgG反应。对6只经皮内（ID）接种梅毒螺旋体和6只经睾丸内（IT）接种梅毒螺旋体的兔血清进行了为期4个月的检测。接种后约第20天，针对大多数梅毒螺旋体表面蛋白（包括那些被认为介导宿主细胞表面黏附的配体）产生了特异性抗体反应。无论接种途径如何，所有动物在第50天时均检测到针对大多数这些免疫原的最大抗体浓度。在选定的兔中观察到了感染IgG反应的差异和相似之处。此外，每只动物的抗体反应性与二期梅毒患者的人血清相当。在梅毒兔血清存在的情况下，通过用含蛋白A的金黄色葡萄球菌对洗涤后的放射性标记生物体进行优先凝集，检测到针对梅毒螺旋体外膜蛋白的抗体。
