Institute of Histology and Embryology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia; International Center for Cardiovascular Diseases MC Medicor d.d., Izola, Slovenia.
International Center for Cardiovascular Diseases MC Medicor d.d., Izola, Slovenia.
Bosn J Basic Med Sci. 2020 May 1;20(2):183-187. doi: 10.17305/bjbms.2019.4409.
Obstructive coronary artery disease (CAD) is characterized by inflammation within the atherosclerotic coronary arteries. Infiltration of inflammatory cells into muscular media can lead to remodeling and weakening of the arterial wall. We examined the relationship between inflammatory infiltration in perivascular adipose tissue (PVAT), state of the external elastic membrane, and the intensity of inflammatory infiltration in the tunica media of coronary arteries obtained by endarterectomy from symptomatic patients with diffuse CAD. We analyzed endarterectomy sequesters from 22 coronary arteries that contained the intima, media, a part of the adventitia, and PVAT in at least one part of the sequester. The coronary arteries were divided into two groups according to the presence or absence of inflammatory infiltration in PVAT. Staining with hematoxylin-eosin and by the Movat's method showed atherosclerotic changes in the intima and media. Immunohistochemistry (anti-leukocyte common antigen [LCA] antibody) was used for the detection of leukocytes. We found a significant positive correlation between inflammatory infiltration in PVAT and preservation of the external elastic membrane of coronary arteries. Furthermore, we found a significant negative correlation between inflammatory infiltration in PVAT and the intensity of inflammatory infiltration in the media. It seems that the integrity of the external elastic membrane and the proinflammatory properties of PVAT restrain inflammatory cells within PVAT. Both effects may prevent the migration of inflammatory cells into the media and delay the development of CAD.
阻塞性冠状动脉疾病 (CAD) 的特征是动脉粥样硬化的冠状动脉内存在炎症。炎症细胞浸润到肌肉中层可导致动脉壁重塑和弱化。我们研究了从弥漫性 CAD 症状患者的动脉内膜切除术获得的血管周围脂肪组织 (PVAT) 中的炎症浸润、外弹性膜状态与冠状动脉中层炎症浸润强度之间的关系。我们分析了 22 个动脉内膜切除术分离物，这些分离物在至少一个分离物部分包含内膜、中层、部分外膜和 PVAT。根据 PVAT 是否存在炎症浸润，将冠状动脉分为两组。苏木精-伊红染色和 Movat 法显示内膜和中层有动脉粥样硬化变化。免疫组织化学（抗白细胞共同抗原 [LCA] 抗体）用于检测白细胞。我们发现 PVAT 中的炎症浸润与冠状动脉外弹性膜的保存之间存在显著的正相关。此外，我们发现 PVAT 中的炎症浸润与中层炎症浸润强度之间存在显著的负相关。看来，外弹性膜的完整性和 PVAT 的促炎特性可限制 PVAT 中的炎症细胞。这两种作用可能阻止炎症细胞迁移到中层，并延缓 CAD 的发展。
