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Cell. 1983 Jul;33(3):865-76. doi: 10.1016/0092-8674(83)90029-6.
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rRNA基因条件性表达后rRNA和tRNA合成的反馈调节以及游离核糖体的积累

Feedback regulation of rRNA and tRNA synthesis and accumulation of free ribosomes after conditional expression of rRNA genes.

作者信息

Gourse R L, Takebe Y, Sharrock R A, Nomura M

出版信息

Proc Natl Acad Sci U S A. 1985 Feb;82(4):1069-73. doi: 10.1073/pnas.82.4.1069.

DOI:10.1073/pnas.82.4.1069
PMID:3156375
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC397195/
Abstract

We have constructed a conditional rRNA gene expression system by fusing a plasmid-encoded rrnB operon to the lambda PL promoter/operator. It was thereby possible to study the events that lead to the regulation of chromosomal rRNA and tRNA synthesis after overproduction of rRNA. rRNA induction resulted in a 2-fold increase in 30S and 50S free ribosomal subunits, whereas the polysome fraction was unaffected. Overproduction of rRNA and "free" ribosomes produced a large repression of rRNA and tRNA synthesis from chromosomal genes and a smaller increase in the concentration of guanosine tetraphosphate. These results lend support to the ribosome feedback regulation model: rRNA and tRNA operons are negatively regulated, either directly or through some intermediate, by free, nontranslating ribosomes.

摘要

我们通过将质粒编码的rrnB操纵子与λPL启动子/操纵基因融合，构建了一个条件性rRNA基因表达系统。借此能够研究rRNA过量产生后导致染色体rRNA和tRNA合成调控的相关事件。rRNA诱导使30S和50S游离核糖体亚基增加了2倍，而多核糖体部分未受影响。rRNA和“游离”核糖体的过量产生导致染色体基因的rRNA和tRNA合成受到强烈抑制，同时鸟苷四磷酸浓度有较小幅度的增加。这些结果支持核糖体反馈调节模型：rRNA和tRNA操纵子受到游离的、未参与翻译的核糖体直接或通过某种中间体进行的负调控。