大肠杆菌在转移到更高温度后RNA合成的调控
Control of RNA synthesis in Escherichia coli after a shift to higher temperature.
作者信息
Ryals J, Little R, Bremer H
出版信息
J Bacteriol. 1982 Sep;151(3):1425-32. doi: 10.1128/jb.151.3.1425-1432.1982.
Abstract
Parameters of RNA synthesis were measured after a temperature upshift in a pair of Escherichia coli B/r strains that are isogenic except for having relA and relA+ loci, to examine the cause for a reported anomaly in the correlation between guanosine tetraphosphate (ppGpp) and stable RNA (rRNA, tRNA) synthesis under such conditions. Two main results were: (i) the specific stable RNA gene activity (stable RNA per total RNA synthesis) correlated in the conventionally expected fashion with the level of ppGpp but was obscured by a nonspecific increase in the RNA chain elongation rate due to the higher temperature; (ii) the temperature upshift caused a transient reduction in the RNA polymerase activity (transcribing per total enzyme) that accounts for the previously observed oscillating RNA synthesis rate after a temperature shift.
摘要
在一对除了具有relA和relA+基因座外其他基因均相同的大肠杆菌B/r菌株中，在温度升高后测量RNA合成参数，以研究在这种条件下鸟苷四磷酸（ppGpp）与稳定RNA（rRNA、tRNA）合成之间报道的异常相关性的原因。两个主要结果是：（i）特定稳定RNA基因活性（每总RNA合成的稳定RNA）以传统预期的方式与ppGpp水平相关，但由于较高温度导致RNA链延伸率非特异性增加而被掩盖；（ii）温度升高导致RNA聚合酶活性（每总酶转录）瞬时降低，这解释了先前观察到的温度变化后RNA合成速率的振荡。
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