环孢素A（CyA）不能促进重型再生障碍性贫血患者的集落形成单位 - 细胞（CFU - c）生长。

Cyclosporin A (CyA) does not enhance CFU-c growth in patients with severe aplastic anaemia.

作者信息

Bacigalupo A, Frassoni F, Podesta' M, Piaggio G, Van Lint M T, Repetto M, Marmont A M

出版信息

Scand J Haematol. 1985 Feb;34(2):133-6. doi: 10.1111/j.1600-0609.1985.tb02245.x.

DOI:10.1111/j.1600-0609.1985.tb02245.x

Abstract

8 patients with severe aplastic anaemia (SAA) in remission following immunosuppressive therapy were studied for CFU-c growth from unfractionated or E-rosette depleted (E-) bone marrow (BM) cells. Cyclosporin A (CyA) was added to unfractionated BM cells at a concentration of 1000 ng/ml. The mean number of CFU-c/10(5) BM cells plated was 6 +/- 6 from unfractionated BM cells, 28 +/- 20 from E-BM cells, and 8 +/- 7 from unfractionated BM cells supplemented with CyA. All patients had significant increase of CFU-c growth after E rosette depletion (overall P = 0.002). On the contrary, only 1 patient showed an increase of CFU-c growth after addition of CyA, and overall there was no difference between untreated and CyA treated BM cells (P = 0.7). These results suggest that addition of CyA to BM cells in vitro is not an effective means of enhancing CFU-c growth in SAA patients.

摘要

对8例接受免疫抑制治疗后处于缓解期的重型再生障碍性贫血（SAA）患者进行了研究，以观察未分离的或经E花环去除（E-）的骨髓（BM）细胞的CFU-c生长情况。将环孢素A（CyA）以1000 ng/ml的浓度添加到未分离的BM细胞中。每10(5)个接种的BM细胞中CFU-c的平均数量，未分离的BM细胞为6±6，E-BM细胞为28±20，添加CyA的未分离BM细胞为8±7。所有患者在E花环去除后CFU-c生长均显著增加（总体P = 0.002）。相反，添加CyA后只有1例患者的CFU-c生长增加，总体而言，未处理的和经CyA处理的BM细胞之间没有差异（P = 0.7）。这些结果表明，体外向BM细胞中添加CyA不是增强SAA患者CFU-c生长的有效方法。