The Key Laboratory of Biomedical Information Engineering, Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, China; Institute for Cancer Research, School of Basic Medical Science, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China.
Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China.
Pathol Res Pract. 2019 Nov;215(11):152621. doi: 10.1016/j.prp.2019.152621. Epub 2019 Sep 3.
Src homology phosphotyrosine phosphatase 2 (Shp2) has been found to be overexpressed in cervical cancer tissues. However, the influence of Shp2 on the biological behavior and sensitivity to cisplatin of cervical cancer cells remains unclear. We aimed to assess Shp2 expression in cervical tissues and cell lines and to detect the influence of Shp2 knockdown and overexpression on the biological behavior and sensitivity to cisplatin in cervical cancer cells. We found that Shp2 expression was significantly upregulated in cervical cancer tissues and cell lines, and Shp2 overexpression was associated with lymph node metastasis and a high human papillomavirus (HPV) DNA load. Shp2 knockdown inhibited cell growth and migration and enhanced sensitivity to cisplatin in the HeLa and SiHa cervical cancer cell lines. In contrast, Shp2 overexpression had the opposite effects. These tumor-promoting effects of Shp2 may be partly related to Akt signaling. In conclusion, Shp2 is involved in the occurrence and development of cervical cancer and may confer cisplatin resistance in cervical cancer. Shp2 blockade may be a new strategy for cervical cancer treatment.
Src 同源磷酸酪氨酸磷酸酶 2（Shp2）在宫颈癌组织中被发现过度表达。然而，Shp2 对宫颈癌细胞的生物学行为和对顺铂的敏感性的影响尚不清楚。我们旨在评估 Shp2 在宫颈组织和细胞系中的表达，并检测 Shp2 敲低和过表达对宫颈癌细胞的生物学行为和对顺铂敏感性的影响。我们发现 Shp2 在宫颈癌组织和细胞系中表达显著上调，Shp2 过表达与淋巴结转移和高人乳头瘤病毒（HPV）DNA 负荷有关。Shp2 敲低抑制 HeLa 和 SiHa 宫颈癌细胞系的细胞生长和迁移，并增强对顺铂的敏感性。相比之下，Shp2 过表达则产生相反的效果。Shp2 的这些促进肿瘤的作用可能部分与 Akt 信号有关。总之，Shp2 参与了宫颈癌的发生和发展，并可能赋予宫颈癌对顺铂的耐药性。Shp2 阻断可能是宫颈癌治疗的一种新策略。
