Armanini D, Strasser T, Weber P C
Am J Physiol. 1985 Mar;248(3 Pt 1):E388-90. doi: 10.1152/ajpendo.1985.248.3.E388.
Aldosterone binding sites in human mononuclear leukocytes were characterized after separation of cells from blood by a Percoll gradient. After washing and resuspension in RPMI-1640 medium, cells were incubated at 37 degrees C for 1 h with different concentrations of [3H]aldosterone plus a 100-fold concentration of RU-26988 (11 alpha, 17 alpha-dihydroxy-17 beta-propynylandrost-1,4,6-trien-3-one), with or without an excess of unlabeled aldosterone. Aldosterone binds to a single class of receptors with an affinity of 2.7 +/- 0.5 nM (means +/- SD, n = 14) and a capacity of 290 +/- 108 sites/cell (n = 14). The specificity data show a hierarchy of affinity of desoxycorticosterone = corticosterone = aldosterone greater than hydrocortisone greater than dexamethasone. The results indicate that mononuclear leukocytes could be useful for studying the physiological significance of these mineralocorticoid receptors and their regulation in humans.
通过Percoll梯度从血液中分离细胞后，对人单核白细胞中的醛固酮结合位点进行了表征。在洗涤并重悬于RPMI-1640培养基中后，将细胞在37℃下与不同浓度的[3H]醛固酮以及100倍浓度的RU-26988（11α，17α-二羟基-17β-丙炔基雄甾-1,4,6-三烯-3-酮）一起孵育1小时，添加或不添加过量的未标记醛固酮。醛固酮以2.7±0.5 nM（平均值±标准差，n = 14）的亲和力和290±108个位点/细胞的容量（n = 14）与单一类别的受体结合。特异性数据显示脱氧皮质酮=皮质酮=醛固酮>氢化可的松>地塞米松的亲和力等级。结果表明，单核白细胞可用于研究这些盐皮质激素受体在人体中的生理意义及其调节。
