Bhandari Dhaka Ram, Coliva Giulia, Fedorova Maria, Spengler Bernhard
Institute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Giessen, Germany.
Faculty of Chemistry and Mineralogy, Institute of Bioanalytical Chemistry, University of Leipzig, Leipzig, Germany.
Methods Mol Biol. 2020;2064:103-111. doi: 10.1007/978-1-4939-9831-9_8.
Complementary to other imaging techniques, MALDI MS imaging provides label-free analysis and chemical specificity, covering a wide range of molecules that can be visualized simultaneously. Here, we highlight a protocol to visualize lipids from single cardiac cells using high-resolution atmospheric-pressure matrix-assisted laser desorption/ionization coupled to an orbital trapping mass analyzer.
作为其他成像技术的补充，基质辅助激光解吸电离质谱成像（MALDI MS成像）提供无标记分析和化学特异性，涵盖可同时可视化的广泛分子。在此，我们重点介绍一种使用与轨道阱质量分析仪联用的高分辨率大气压基质辅助激光解吸/电离技术对单个心肌细胞中的脂质进行可视化的方案。
