Wambebe C
Br J Pharmacol. 1985 Jan;84(1):185-91.
The effects of 5-hydroxytryptophan (5-HTP), citalopram, p-chlorophenylalanine (PCPA), cyproheptadine, lysergic acid diethylamide (LSD-25), metitepine and NSD 1034 on nitrazepam-induced sleep were investigated in mice. Nitrazepam (1.6-25.6 mg kg-1, i.p.) induced a dose-dependent sedative-hypnotic effect. 5-HTP (8-128 mg kg-1 i.m.) did not induce behavioural sleep but sedated mice and significantly potentiated nitrazepam-induced sleep. Similarly, 5-HTP (4-32 mg kg-1, i.m.) increased pentobarbitone sleeping time. Citalopram (2.5-10 mg kg-1, i.p.) significantly potentiated nitrazepam sleep. PCPA (300-400 mg kg-1, i.p.) completely abolished nitrazepam sleep; 5-HTP (32 mg kg-1, i.m.) reversed this effect. NSD 1034 (75-150 mg kg-1, i.p.) antagonized the potentiating effect of 5-HTP (32 mg kg-1, i.m.) on nitrazepam sleep. Cyproheptadine (5-10 mg kg-1, i.p.) and LSD-25 (2.5-10 micrograms kg-1, i.p.) partially antagonized nitrazepam sleep. Similarly, 5-HTP-induced potentiation of nitrazepam sleep was antagonized by cyproheptadine and LSD-25. Metitepine (4-8 mg kg-1, i.p.) induced behavioural sleep and significantly potentiated nitrazepam sleep. Ro15-1788 (10 mg kg-1, i.p.) effectively antagonized nitrazepam-induced sleep. These results indicate that enhancement of central 5-HT neurotransmission may underlie nitrazepam-induced sleep in mice.
研究了5-羟色氨酸（5-HTP）、西酞普兰、对氯苯丙氨酸（PCPA）、赛庚啶、麦角酸二乙酰胺（LSD-25）、米氮平及NSD 1034对小鼠硝西泮诱导睡眠的影响。硝西泮（1.6 - 25.6 mg kg-1，腹腔注射）诱导出剂量依赖性的镇静催眠作用。5-HTP（8 - 128 mg kg-1，肌肉注射）未诱导行为性睡眠，但使小鼠镇静并显著增强硝西泮诱导的睡眠。同样，5-HTP（4 - 32 mg kg-1，肌肉注射）延长了戊巴比妥睡眠时间。西酞普兰（2.5 - 10 mg kg-1，腹腔注射）显著增强硝西泮睡眠。PCPA（300 - 400 mg kg-1，腹腔注射）完全消除硝西泮睡眠；5-HTP（32 mg kg-1，肌肉注射）逆转了这种作用。NSD 1034（75 - 150 mg kg-1，腹腔注射）拮抗5-HTP（32 mg kg-1，肌肉注射）对硝西泮睡眠的增强作用。赛庚啶（5 - 10 mg kg-1，腹腔注射）和LSD-25（2.5 - 10 μg kg-1，腹腔注射）部分拮抗硝西泮睡眠。同样，赛庚啶和LSD-25拮抗5-HTP诱导的硝西泮睡眠增强作用。米氮平（4 - 8 mg kg-1，腹腔注射）诱导行为性睡眠并显著增强硝西泮睡眠。Ro15 - 1788（10 mg kg-1，腹腔注射）有效拮抗硝西泮诱导的睡眠。这些结果表明，中枢5-羟色胺神经传递的增强可能是小鼠硝西泮诱导睡眠的基础。
