Praxedes Érika A, de Queiroz Neta Luiza B, Borges Alana A, Silva Maria B, Santos Maria V O, Ribeiro Leandro R, Silva Herlon V R, Pereira Alexsandra F
Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Mossoro, Brazil.
Aba-Yby Conservation Institute, Environmental Park and Zoo Ecopoint, Fortaleza, Brazil.
Anat Histol Embryol. 2020 Jan;49(1):121-129. doi: 10.1111/ahe.12500. Epub 2019 Sep 30.
Skin of mammals vulnerable to extinction, such as the jaguar, is used as a source of material in conservation strategies. The composition of skin is not uniform among species, and the ability to distinguish similarities in skin morphology in animal groups is fundamental in the application of skin tissue for use in biobanks. The aim of our study was to evaluate the structure, composition and capacity for culture of ear skin from the yellow and black jaguars. Both qualitative and quantitative methods were used, focusing on skin thickness, cell quantification and distribution, collagen density, proliferative activity and viability. Histomorphometrical study of the skin showed a total thickness of 273.2 and 274.6 µm for the yellow and black jaguars, respectively. Melanocytes and fibroblasts were, respectively, 9.7 and 23.0 for the yellow jaguar and 11.3 and 26.8 for the black jaguar. A collagen density of 67.0% and 49.0% was observed for yellow and black jaguars, respectively. Both animals presented a proliferative activity varying between 1.20 and 1.30. All tissues could promote cellular detachment, reaching subconfluence in 10-15 days. This kind of information from histomorphometrical features and cell cultures can be essential for a more targeted application of ear skin cryopreservation in this species, as such information will enable understanding the action of substances on tissues during the conservation process.
诸如美洲豹等易灭绝哺乳动物的皮肤被用作保护策略中的材料来源。不同物种的皮肤组成并不一致，而在动物群体中辨别皮肤形态相似性的能力对于将皮肤组织应用于生物样本库至关重要。我们研究的目的是评估黄黑美洲豹耳部皮肤的结构、组成及培养能力。我们采用了定性和定量方法，重点关注皮肤厚度、细胞定量与分布、胶原蛋白密度、增殖活性及活力。皮肤的组织形态计量学研究表明，黄美洲豹和黑美洲豹皮肤的总厚度分别为273.2微米和274.6微米。黄美洲豹的黑素细胞和成纤维细胞数量分别为9.7和23.0，黑美洲豹则分别为11.3和26.8。黄黑美洲豹的胶原蛋白密度分别为67.0%和49.0%。两种动物的增殖活性均在1.20至1.30之间变化。所有组织均可促使细胞脱离，在10 - 15天内达到亚汇合状态。来自组织形态计量学特征和细胞培养的此类信息对于该物种耳部皮肤冷冻保存更具针对性的应用可能至关重要，因为这些信息将有助于理解保存过程中物质对组织的作用。
