Department of Physical Therapy, Universidade Federal do Pampa (Unipampa), Uruguaiana, Rio Grande do Sul, Brazil.
Laboratory of Experimental Neuroscience (LaNEx), Universidade do Sul de Santa Catarina (UNISUL), Palhoça, Santa Catarina, Brazil.
J Biophotonics. 2020 Jan;13(1):e201900204. doi: 10.1002/jbio.201900204. Epub 2019 Oct 15.
Knee osteoarthritis (OA) is a chronic disease that causes pain and gradual degeneration of the articular cartilage. In this study, MIA-induced OA knee model was used in rats to test the effects of the photobiomodulation therapy (PBM). We analyzed the inflammatory process (pain and cytokine levels), and its influence on the oxidative stress and antioxidant capacity. Knee OA was induced by monosodium iodoacetate (MIA) intra-articular injection (1.5 mg/50 μL) and the rats were treated with eight sessions of PBM 3 days/week (904 nm, 6 or 18 J/cm ). For each animal, mechanical and cold hyperalgesia and spontaneous pain were evaluated; biological analyses were performed in blood serum, intra-articular lavage, knee structures, spinal cord and brainstem. Cytokine assays were performed in knee, spinal cord and brainstem samples. The effects of the 18 J/cm dose of PBM were promising in reducing pain and neutrophil activity in knee samples, together with reducing oxidative stress damage in blood serum and spinal cord samples. PBM improved the antioxidant capacity in blood serum and brainstem, and decreased the knee pro-inflammatory cytokine levels. Our study demonstrated that PBM decreased oxidative damage, inflammation and pain. Therefore, this therapy could be an important tool in the treatment of knee OA.
膝关节骨关节炎（OA）是一种慢性疾病，会导致关节软骨疼痛和逐渐退化。在这项研究中，使用 MIA 诱导的 OA 膝关节模型在大鼠中测试光生物调节疗法（PBM）的效果。我们分析了炎症过程（疼痛和细胞因子水平）及其对氧化应激和抗氧化能力的影响。通过关节内注射单碘乙酸盐（MIA）（1.5 mg/50 μL）诱导膝关节 OA，每周 3 天给予大鼠 8 次 PBM 治疗（904nm，6 或 18 J/cm）。对于每只动物，评估机械和冷痛觉过敏以及自发性疼痛；在血清、关节内灌洗、膝关节结构、脊髓和脑干中进行生物学分析。在膝关节、脊髓和脑干样本中进行细胞因子测定。PBM 对减轻膝关节疼痛和中性粒细胞活性以及降低血清和脊髓样本的氧化应激损伤有显著效果。PBM 提高了血清和脑干的抗氧化能力，并降低了膝关节促炎细胞因子水平。我们的研究表明，PBM 可减少氧化损伤、炎症和疼痛。因此，这种疗法可能是治疗膝关节 OA 的重要手段。
