Graduate Program in Biochemistry, Universidade Federal do Pampa (Unipampa), BR 472, Km 585, Uruguaiana, RS, 97501-970, Brazil.
Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analyses, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil.
Lasers Med Sci. 2022 Feb;37(1):193-204. doi: 10.1007/s10103-020-03207-8. Epub 2021 Jan 8.
The aim of this study was to assess potential combination effects of photobiomodulation therapy (PBMT) with Sida tuberculata extracts on the oxidative stress and antioxidant activity, as well as on the inflammatory process. Rats with knee osteoarthritis (OA) were treated with S. tuberculata extracts and PBMT (904 nm, 18 J/cm). The animals were evaluated for nociception and edema. The blood, knee lavage and structures, spinal cord, and brainstem were collected for biochemical analyses (lipid peroxidation, protein carbonyl content, superoxide dismutase activity, non-protein thiol levels, and measurement of nitrite/nitrate). The knee structures were also used to measure cytokine levels. PBMT lowered the damage due to oxidative stress in the knee and at distant sites from the lesion. PBMT also reduced the levels of nitric oxide and cytokines, which could explain the nociception reduction mechanism. Similarly, S. tuberculata decreased the damage by oxidative stress, levels of nitrite/nitrate, and cytokines. The therapy combination reduced levels of cytokines and nitrite/nitrate. PBMT and S. tuberculata extracts reduced the oxidative stress and inflammation. It is noteworthy that PBMT increased the antioxidant activity in the knee and at sites distant from the lesion, contributing to a more significant decrease in nociception. The combination of therapies did not present significant effects on the analyzed parameters. Therefore, it is suggested that PBM is sufficient to minimize the signs and symptoms of the knee OA in our rat model.
本研究旨在评估光生物调节疗法（PBMT）与菝葜提取物联合应用对氧化应激和抗氧化活性以及炎症过程的潜在协同作用。膝骨关节炎（OA）大鼠接受菝葜提取物和 PBMT（904nm，18J/cm）治疗。通过评估动物的疼痛和水肿来评价治疗效果。采集血液、膝关节灌洗液和结构、脊髓和脑干进行生化分析（脂质过氧化、蛋白羰基含量、超氧化物歧化酶活性、非蛋白巯基水平以及亚硝酸盐/硝酸盐的测量）。还对膝关节结构进行了细胞因子水平的测量。PBMT 降低了膝关节和远离病变部位的氧化应激损伤。PBMT 还降低了一氧化氮和细胞因子的水平，这可以解释其镇痛作用的机制。类似地，菝葜提取物通过降低氧化应激、亚硝酸盐/硝酸盐和细胞因子的水平减轻了损伤。联合治疗降低了细胞因子和亚硝酸盐/硝酸盐的水平。PBMT 和菝葜提取物均能减轻氧化应激和炎症。值得注意的是，PBMT 增加了膝关节和远离病变部位的抗氧化活性，有助于更显著地减轻疼痛。两种治疗方法对分析参数均无显著影响。因此，建议 PBMT 足以减轻我们的大鼠模型中膝骨关节炎的症状和体征。
