Yang V K, Rush J E, Bhasin S, Wagers A J, Lee R T
Department of Clinical Sciences, Cummings School of Veterinary Medicine at Tufts University, 200 Westboro Rd, North Grafton, MA, 01536, USA.
Department of Clinical Sciences, Cummings School of Veterinary Medicine at Tufts University, 200 Westboro Rd, North Grafton, MA, 01536, USA.
J Vet Cardiol. 2019 Oct;25:41-51. doi: 10.1016/j.jvc.2019.08.002. Epub 2019 Sep 1.
Growth differentiation factor (GDF) 11 has been shown to reduce cardiac hypertrophy in mice. Low levels of GDF-11 are associated with cardiac hypertrophy in humans. The authors hypothesized that plasma GDF-11 level is decreased in cats with hypertrophic cardiomyopathy (HCM). Given the close homology between GDF-11 and myostatin/GDF-8, GDF-8 levels were also assessed.
Thirty-seven client-owned cats were enrolled, including cats with normal cardiac structure (n = 16), cats with HCM or hypertrophic obstructive cardiomyopathy (HOCM; n = 14), and cats with HCM and congestive heart failure (CHF; n = 7).
Plasma samples were analyzed for GDF-8 and GDF-11 using liquid chromatography tandem-mass spectrometry. Levels of GDF-8 and GDF-11 were compared between cats with normal cardiac structure, HCM or HOCM, and CHF.
No differences in GDF-11 concentrations were found between cats with normal cardiac structure and cats with HCM/HOCM, with or without history of CHF. Decreased GDF-8 concentrations were detected in cats with CHF compared to cats with HCM/HOCM without history of CHF (p=0.031) and cats with normal cardiac structure (p=0.027). Growth differentiation factor 8 was higher in cats with HOCM compared to those with CHF (p=0.002). No statistical difference was noted in GDF-8 level as a function of age, weight, or body condition score.
Plasma GDF-11 was not different between cats with HCM/HOCM and cats with normal cardiac structure regardless of age. Plasma GDF-8 was decreased in cats with CHF compared to cats with normal cardiac structure and cats with asymptomatic HCM/HOCM, suggesting a possible role in CHF development.
生长分化因子（GDF）11已被证明可减轻小鼠的心脏肥大。人类中GDF-11水平较低与心脏肥大有关。作者推测肥厚型心肌病（HCM）猫的血浆GDF-11水平会降低。鉴于GDF-11与肌肉生长抑制素/GDF-8之间的密切同源性，还评估了GDF-8水平。
纳入37只客户拥有的猫，包括心脏结构正常的猫（n = 16）、患有HCM或肥厚性梗阻性心肌病（HOCM；n = 14）的猫以及患有HCM和充血性心力衰竭（CHF；n = 7）的猫。
使用液相色谱串联质谱法分析血浆样本中的GDF-8和GDF-11。比较心脏结构正常的猫、患有HCM或HOCM的猫以及患有CHF的猫之间GDF-8和GDF-11的水平。
心脏结构正常的猫与患有HCM/HOCM的猫（无论有无CHF病史）之间，GDF-11浓度没有差异。与无CHF病史的HCM/HOCM猫（p = 0.031）和心脏结构正常的猫（p = 0.027）相比，CHF猫的GDF-8浓度降低。与CHF猫相比，HOCM猫的生长分化因子8更高（p = 0.002）。未发现GDF-8水平随年龄、体重或身体状况评分有统计学差异。
无论年龄如何，HCM/HOCM猫与心脏结构正常的猫之间血浆GDF-11没有差异。与心脏结构正常的猫和无症状HCM/HOCM猫相比，CHF猫的血浆GDF-8降低，提示其在CHF发展中可能起作用。
