三重免疫层析系统用于同时检测牛布鲁氏菌病、结核病和白血病的血清学诊断。
Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia.
机构信息
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, Moscow 119071, Russia.
Republican State Enterprise "National Center for Biotechnology" under the Science Committee of Ministry of Education and Science of the Republic of Kazakhstan, Nursultan 010000, Kazakhstan.
出版信息
Biosensors (Basel). 2019 Sep 29;9(4):115. doi: 10.3390/bios9040115.
An immunochromatographic test system has been developed for the simultaneous rapid multiplex serodiagnostics of bovine brucellosis, tuberculosis, and leukemia. The test system is based on the use of a conjugate of gold nanoparticles with the chimeric protein Cysteine-A/G and three analytical zones with immobilized pathogen antigens: lipolysaccharide, recombinant proteins MPB64 and MPB83-MPB63 of , and recombinant protein p24 of the bovine leukemia virus. Prototypes of the test system were tested on 98 samples of sera from healthy and infected animals. The diagnostic sensitivity of the developed test system was 92% for brucellosis, 92% for tuberculosis, and 96% for leukemia. False positive test results were not observed.
已开发出一种免疫层析测试系统，用于同时快速多重检测牛布鲁氏菌病、结核病和白血病的血清抗体。该测试系统基于使用金纳米粒子与嵌合蛋白半胱氨酸-A/G 的缀合物和三个带有固定病原体抗原的分析区：脂多糖、牛分枝杆菌的重组蛋白 MPB64 和 MPB83-MPB63，以及牛白血病病毒的重组蛋白 p24。该测试系统的原型在 98 份来自健康和感染动物的血清样本上进行了测试。该测试系统对布鲁氏菌病的诊断灵敏度为 92%，对结核病的诊断灵敏度为 92%，对白血病的诊断灵敏度为 96%。未观察到假阳性测试结果。
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