Wu Hong, Lei Zhen, Gao Shuibo, Dai Liping, Han Yongjun, Gao Haixia, Wang Xinzhou, Wang Zhentao, Han Lihua
Laboratory of Cell Imaging.
Institute of Cardiovascular Disease.
Acta Cardiol Sin. 2019 Sep;35(5):524-533. doi: 10.6515/ACS.201909_35(5).20190210A.
YiqiHuoxue decoction (YHD) is frequently prescribed to prevent and treat cardiovascular diseases. YHD inhibits platelet aggregation, however the underlying mechanisms are unclear.
The and anti-platelet and antithrombotic effects of YHD and ethanol-precipitated YHD (EYHD) and underlying mechanisms were investigated. Forty-six Sprague-Dawley (SD) rats and 36 male Kunming mice were examined. Ten SD rats were used to assess the cytotoxicity of YHD and EYHD by releasing lactate dehydrogenase from treated platelets. The remaining 36 SD rats were divided into six groups (six per group), including control saline (5 mL/kg), aspirin (20 mg/kg), YHD low dosage (0.2 g/kg), YHD high dosage (2.0 g/kg), 75% EYHD low dosage (0.2 g/kg), and 75% EYHD high dosage (2.0 g/kg) groups to detect platelet aggregation; the 36 Kunming mice were divided into 6 groups to detect mesenteric arterial thrombosis induction. Thromboxane B2 (TXB2) levels were determined by enzyme immunoassay.
YHD high dosage and 75% EYHD (low and high dosage) inhibited ADP-induced platelet aggregation. Moreover, collagen-induced platelet aggregation was significantly suppressed by YHD (high dosage), 75% EYHD (high dosage), and 75% EYHD (low dose). Rats given 75% EYHD (high dose) displayed a marked reduction in collagen-induced platelet aggregation at 2 h post-administration. YHD and EYHD markedly prolonged the onset of thrombosis causing loose attachment of the thrombus to the vascular endothelium, but bleeding and clotting times were not significantly changed. Finally, YHD and EYHD markedly reduced TXB2 levels.
YHD and EYHD effectively inhibit platelet activation and thrombosis, presumably by suppressing TXB2.
益气活血汤（YHD）常用于预防和治疗心血管疾病。YHD可抑制血小板聚集，但其潜在机制尚不清楚。
研究了YHD及其乙醇沉淀产物（EYHD）的抗血小板和抗血栓作用及其潜在机制。对46只Sprague-Dawley（SD）大鼠和36只雄性昆明小鼠进行了检测。10只SD大鼠用于通过检测处理后的血小板释放乳酸脱氢酶来评估YHD和EYHD的细胞毒性。其余36只SD大鼠分为6组（每组6只），包括对照生理盐水（5 mL/kg）、阿司匹林（20 mg/kg）、YHD低剂量（0.2 g/kg）、YHD高剂量（2.0 g/kg）、75% EYHD低剂量（0.2 g/kg）和75% EYHD高剂量（2.0 g/kg）组，以检测血小板聚集；36只昆明小鼠分为6组以检测肠系膜动脉血栓形成。通过酶免疫测定法测定血栓素B2（TXB2）水平。
YHD高剂量和75% EYHD（低剂量和高剂量）抑制ADP诱导的血小板聚集。此外，YHD（高剂量）、75% EYHD（高剂量）和75% EYHD（低剂量）显著抑制胶原诱导的血小板聚集。给予75% EYHD（高剂量）的大鼠在给药后2小时胶原诱导的血小板聚集明显减少。YHD和EYHD显著延长血栓形成时间，使血栓与血管内皮的附着松散，但出血和凝血时间无明显变化。最后，YHD和EYHD显著降低TXB2水平。
YHD和EYHD可能通过抑制TXB2有效抑制血小板活化和血栓形成。
