Fu Ying-Jie, Yan Yu-Qi, Zheng Xiao, Shi Shan-Shan, Wu Sha, Jiang Zhen-You
1Department of Microbiology and Immunology, School of Basic Medical Sciences, Jinan University, Guangzhou, 510632 Guangdong China.
2Institute of Medical Microbiology, Jinan University, Guangzhou, 510632 Guangdong China.
Chin Med. 2019 Sep 27;14:39. doi: 10.1186/s13020-019-0256-7. eCollection 2019.
To investigate the effects and immunological mechanisms of the traditional Chinese medicine Xinjiaxiangruyin on controlling influenza virus (FM1 strain) infection in mice housed in a hygrothermal environment.
Mice were housed in normal and hygrothermal environments, and intranasally infected with influenza virus (FM1). A high-performance liquid chromatography fingerprint of Xinjiaxiangruyin was used to provide an analytical method for quality control. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to measure messenger RNA expression of Toll-like receptor 7 (TLR7), myeloid differentiation primary response 88 (MyD88), and nuclear factor-kappa B (NF-κB) p65 in the TLR7 signaling pathway and virus replication in the lungs. Western blotting was used to measure the expression levels of TLR7, MyD88, and NF-κB p65 proteins. Flow cytometry was used to detect the proportion of Th17/T-regulatory cells.
Xinjiaxiangruyin effectively alleviated lung inflammation in C57BL/6 mice in hot and humid environments. Guizhimahuanggebantang significantly reduced lung inflammation in C57BL/6 mice. The expression of TLR7, MyD88, and NF-κB p65 mRNA in lung tissue of WT mice in the normal environment, GZMHGBT group was significantly lower than that in the model group (P < 0.05). In WT mice exposed to the hot and humid environment, the expression levels of TLR7, MyD88, and NF-κB p65 mRNA in the XJXRY group were significantly different from those in the virus group. The expression levels of TLR7, MyD88, and NF-κB p65 protein in lung tissue of WT mice exposed to the normal environment, GZMHGBT group was significantly lower than those in the model group. In WT mice exposed to hot and humid environments, the expression levels of TLR7, MyD88, and NF-κB p65 protein in XJXRY group were significantly different from those in the virus group.
Guizhimahuanggebantang demonstrated a satisfactory therapeutic effect on mice infected with the influenza A virus (FM1 strain) in a normal environment, and Xinjiaxiangruyin demonstrated a clear therapeutic effect in damp and hot environments and may play a protective role against influenza through downregulation of the TLR7 signal pathway.
研究中药新加香薷饮对湿热环境下小鼠感染流感病毒（FM1株）的影响及免疫机制。
将小鼠饲养于正常和湿热环境中，经鼻感染流感病毒（FM1）。采用新加香薷饮高效液相色谱指纹图谱提供质量控制分析方法。采用实时定量聚合酶链反应（RT-qPCR）检测Toll样受体7（TLR7）、髓样分化初级反应88（MyD88）和核因子-κB（NF-κB）p65在TLR7信号通路中的信使核糖核酸表达以及肺组织中病毒复制情况。采用蛋白质免疫印迹法检测TLR7、MyD88和NF-κB p65蛋白的表达水平。采用流式细胞术检测Th17/调节性T细胞的比例。
新加香薷饮有效减轻了湿热环境下C57BL/6小鼠的肺部炎症。桂枝麻黄各半汤显著减轻了C57BL/6小鼠的肺部炎症。正常环境下野生型（WT）小鼠肺组织中，桂枝麻黄各半汤组TLR7、MyD88和NF-κB p65信使核糖核酸的表达显著低于模型组（P < 0.05）。在暴露于湿热环境的WT小鼠中，新加香薷饮组TLR7、MyD88和NF-κB p65信使核糖核酸的表达水平与病毒组显著不同。正常环境下野生型小鼠肺组织中，桂枝麻黄各半汤组TLR7、MyD88和NF-κB p65蛋白的表达显著低于模型组。在暴露于湿热环境的WT小鼠中，新加香薷饮组TLR7、MyD88和NF-κB p65蛋白的表达水平与病毒组显著不同。
桂枝麻黄各半汤对正常环境下感染甲型流感病毒（FM1株）的小鼠显示出满意的治疗效果，新加香薷饮在湿热环境下显示出明显的治疗效果，可能通过下调TLR7信号通路对流感起到保护作用。
