Zanaboni Moira, Roda Gabriella, Arnoldi Sebastiano, Casagni Eleonora, Gambaro Veniero, Dei Cas Michele
DANI Instruments, Viale Brianza 87, 20093, Cologno Monzese, Italy.
Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano, via Mangiagalli 25, Milan, 20133, Italy.
J Forensic Sci. 2020 Mar;65(2):636-640. doi: 10.1111/1556-4029.14206. Epub 2019 Oct 1.
The determination of carbon monoxide (CO) and carboxyhemoglobin (COHb) is of utmost importance in forensic toxicology to determine the cause of death in cases of CO poisoning, fire, and explosions. To this end, reliable and updated analytical methods are required. In this paper, four different methods for the determination of carbon monoxide in postmortem blood samples were compared: (i) the spectrophotometric determination of COHb applying the method proposed by Rodkey and modified by Beutler-West, (ii) the spectrophotometric determination of CO using a micro-diffusion-based method, (iii) the determination of CO by gas chromatography coupled to a TCD detector, and (iv) the determination of COHb by blood gas analysis. Three postmortem blood samples were analyzed with all methods, and the results were comparable. The applied methodologies showed different features depending on the sensitivity, sample preparation, and volume. The HS-GC/TCD method in our hand was the most appropriate, on postmortem samples, and versatile to apply. Unfortunately, only a limited number of postmortem blood samples were available for this study due to the rarity of that kind of intoxication in our jurisdiction.
在法医毒理学中，测定一氧化碳（CO）和碳氧血红蛋白（COHb）对于确定一氧化碳中毒、火灾和爆炸案件中的死因至关重要。为此，需要可靠且最新的分析方法。本文比较了四种不同的测定死后血液样本中一氧化碳的方法：（i）采用Rodkey提出并经Beutler-West修改的方法进行分光光度法测定COHb；（ii）使用基于微扩散的方法进行分光光度法测定CO；（iii）通过与热导检测器联用的气相色谱法测定CO；（iv）通过血气分析测定COHb。使用所有方法对三个死后血液样本进行了分析，结果具有可比性。所应用的方法根据灵敏度、样品制备和体积表现出不同的特点。我们采用的顶空-气相色谱/热导检测器方法对于死后样本最为合适且应用广泛。不幸的是，由于我们辖区内此类中毒事件罕见，本研究仅获得了有限数量的死后血液样本。
