Department of Pharmaceutics, PES's Modern College of Pharmacy, Sector No. 21, Yamuna Nagar, Nigdi, Pune, (M.S), India.
Department of Pharmaceutics, RMD Institute of Pharmaceutical Education & Research, Pune, (M.S), India.
IET Nanobiotechnol. 2019 Sep;13(7):688-696. doi: 10.1049/iet-nbt.2018.5421.
The motive of work was to develop a multi-walled carbon nanoplatform through facile method for transportation of potential anticancer drug doxorubicin (DOX). Folic acid (FA)-ethylene diamine (EDA) anchored and acid functionalised MWCNTs were covalently grafted with DOX via π-π stacking interaction. The resultant composite was corroborated by H NMR, FTIR, XRD, EDX, SEM, and DSC study. The drug entrapment efficiency of FA-conjugated MWCNT was found high and stability study revealed its suitability in biological system. FA-EDA-MWCNTs-DOX conjugate demonstrated a significant anticancer activity on human breast cancer MCF-7 cells. MTT study revealed the lesser cytotoxicity of folate-conjugated MWCNTs. The obtained results demonstrated the targeting specificity of FA-conjugate via overexpressed folate receptor deemed greater scientific value to overcome multidrug protection during cancer therapy. The proposed strategy is a gentle contribution towards development of biocompatible targeted drug delivery and offers potential to address the current challenges in cancer therapy.
目的是通过简便的方法开发一种多壁碳纳米平台，用于输送潜在的抗癌药物阿霉素（DOX）。叶酸（FA）-乙二胺（EDA）锚定和酸功能化的 MWCNTs 通过π-π堆积相互作用与 DOX 共价接枝。通过 H NMR、FTIR、XRD、EDX、SEM 和 DSC 研究证实了所得复合材料。FA 修饰的 MWCNT 的药物包封效率高，稳定性研究表明其适用于生物体系。FA-EDA-MWCNTs-DOX 缀合物对人乳腺癌 MCF-7 细胞表现出显著的抗癌活性。MTT 研究表明，叶酸修饰的 MWCNTs 的细胞毒性较小。研究结果表明，通过过表达叶酸受体，FA 缀合物具有靶向特异性，这为克服癌症治疗中多药保护提供了更大的科学价值。所提出的策略是朝着开发生物相容性靶向药物传递的温和贡献，并提供解决癌症治疗中当前挑战的潜力。
