Institute of Biomedicine and Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, P.R. China.
Department of Stomatology, Jinan University Medical College, Guangzhou, P.R. China.
J Periodontol. 2020 Jul;91(7):975-985. doi: 10.1002/JPER.19-0273. Epub 2020 Jan 16.
Periodontal ligament stem cells (PDLSCs) play an essential role in periodontal tissue repair. Basic fibroblast growth factor (bFGF) has been used in the clinical treatment of periodontal disease. However, studies have shown that bFGF inhibits the osteogenic differentiation of PDLSCs, which is not conducive to alveolar bone repair. Sulfonated chitosan oligosaccharide (SCOS), a heparan-like compound, can maintain the conformation of bFGF and promote its proliferation activity. This study investigated the effects of bFGF in combination with SCOS on the osteogenic differentiation of hPDLSCs.
hPDLSCs were isolated from healthy human periodontal ligament and identified by flow cytometry and immunofluorescence. The affinity between SCOS and bFGF was analyzed by surface plasmon resonance. Changes in osteogenic differentiation by combination of bFGF with SCOS were analyzed by alkaline phosphatase activity assay, Sirius Red staining, and Alizarin Red staining. Expression of genes and proteins was investigated by western blotting and reverse transcription-quantitative PCR.
Extracted hPDLSCs were mesenchymal stem cells with pluripotent differentiation potential. SCOS exhibited an affinity for bFGF. bFGF (20 ng/mL) promoted the proliferation of hPDLSCs, but inhibited their osteogenic differentiation. SCOS alleviated the inhibitory effect of bFGF on the osteogenic differentiation of hPDLSCs.
SCOS can reduce the inhibitory effect of bFGF on the osteogenic differentiation of hPDLSCs. This study provides evidence for the clinical use of bFGF to repair periodontal tissue.
牙周膜干细胞（PDLSCs）在牙周组织修复中发挥着重要作用。碱性成纤维细胞生长因子（bFGF）已被用于牙周病的临床治疗。然而，研究表明 bFGF 抑制 PDLSCs 的成骨分化，不利于牙槽骨修复。磺化壳聚糖寡糖（SCOS）是一种类似肝素的化合物，能够维持 bFGF 的构象并促进其增殖活性。本研究探讨了 bFGF 与 SCOS 联合对人牙周膜干细胞（hPDLSCs）成骨分化的影响。
从健康人牙周膜中分离 hPDLSCs，通过流式细胞术和免疫荧光鉴定。通过表面等离子体共振分析 SCOS 与 bFGF 的亲和力。通过碱性磷酸酶活性测定、茜素红染色和茜素红染色分析 bFGF 与 SCOS 联合对成骨分化的影响。通过 Western blot 和逆转录定量 PCR 研究基因和蛋白的表达。
提取的 hPDLSCs 是具有多能分化潜能的间充质干细胞。SCOS 对 bFGF 具有亲和力。bFGF（20ng/mL）促进 hPDLSCs 的增殖，但抑制其成骨分化。SCOS 减轻了 bFGF 对 hPDLSCs 成骨分化的抑制作用。
SCOS 可减轻 bFGF 对 hPDLSCs 成骨分化的抑制作用。本研究为临床应用 bFGF 修复牙周组织提供了依据。
