Department of Chemistry, University of Kansas, Lawrence, KS 66045, USA.
Sensors (Basel). 2023 May 17;23(10):4831. doi: 10.3390/s23104831.
The Nicoya OpenSPR is a benchtop surface plasmon resonance (SPR) instrument. As with other optical biosensor instruments, it is suitable for the label-free interaction analysis of a diverse set of biomolecules, including proteins, peptides, antibodies, nucleic acids, lipids, viruses, and hormones/cytokines. Supported assays include affinity/kinetics characterization, concentration analysis, yes/no assessment of binding, competition studies, and epitope mapping. OpenSPR exploits localized SPR detection in a benchtop platform and can be connected with an autosampler (XT) to perform automated analysis over an extended time period. In this review article, we provide a comprehensive survey of the 200 peer-reviewed papers published between 2016 and 2022 that use the OpenSPR platform. We highlight the range of biomolecular analytes and interactions that have been investigated using the platform, provide an overview on the most common applications for the instrument, and point out some representative research that highlights the flexibility and utility of the instrument.
尼科亚开放式表面等离子体共振（SPR）仪是一种台式表面等离子体共振（SPR）仪器。与其他光学生物传感器仪器一样，它适用于多种生物分子（包括蛋白质、肽、抗体、核酸、脂质、病毒以及激素/细胞因子）的无标记相互作用分析。支持的检测方法包括亲和力/动力学特性分析、浓度分析、结合的是/否评估、竞争研究和表位作图。开放式 SPR 在台式平台中利用局部 SPR 检测，并可与自动进样器（XT）连接，以在较长时间段内进行自动化分析。在这篇综述文章中，我们全面调查了 2016 年至 2022 年间发表的 200 篇使用开放式 SPR 平台的同行评议论文。我们重点介绍了使用该平台研究的各种生物分子分析物和相互作用，概述了仪器的最常见应用，并指出了一些具有代表性的研究，突出了仪器的灵活性和实用性。
