Toxicology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695 012, Kerala, India.
Toxicology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695 012, Kerala, India.
Colloids Surf B Biointerfaces. 2019 Dec 1;184:110530. doi: 10.1016/j.colsurfb.2019.110530. Epub 2019 Sep 24.
Fullerene C70 with symmetric nanostructure and unique properties that open up leeway for both material science and healthcare applications. Poor water dispersity and limited knowledge about its associated toxicity hinders the biomedical scope of C70. These restraining factors need to be addressed. Dextran, natural and water-soluble polymer was used to improve the dispersity of C70 in water. Dextran coating on C70 successfully yielded stable dispersion of C70 in water with remarkable cytocompatibility with L929 fibroblast cells. The dextran-coated C70 was characterized using different characterization techniques such as fourier transformed-infrared spectroscopy (FT-IR), transition electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential. The cell viability assays suggested that the L929 cells retained more than 80% cell viability after 24 h treatment with dextran-coated C70. The mitochontrial membrane potential of the treated cells were found to be uncompromised. Fluorescent imaging techniques done with the aim of evaluating the integrity of lysosomes detected no potential toxicity in L929 cells treated with dextran-coated C70. Actin filaments showed intact organelles when viewed using Rhodamine- phalloidin staining after 24 h post-treatment. DAPI staining also revealed the integrity of nucleus after exposure to dextran-coated C70. The calcein AM/PI flow cytometry analysis further confirmed that dextran-coated C70 kept cell viability of treated cells above 80% for all concentrations. The results points out the scope of dextran-coated C70 for various health care applications.
具有对称纳米结构和独特性质的富勒烯 C70，为材料科学和医疗保健应用开辟了广阔的空间。较差的水分散性和对其相关毒性的有限了解，限制了 C70 在生物医学领域的应用。这些制约因素需要得到解决。葡聚糖是一种天然的、水溶性聚合物，用于提高 C70 在水中的分散性。C70 表面的葡聚糖涂层成功地使 C70 在水中稳定分散，并且与 L929 成纤维细胞具有显著的细胞相容性。使用不同的表征技术，如傅里叶变换红外光谱（FT-IR）、透射电子显微镜（TEM）、动态光散射（DLS）和zeta 电位对葡聚糖包覆的 C70 进行了表征。细胞活力测定表明，L929 细胞在经过 24 小时葡聚糖包覆的 C70 处理后，细胞活力保持在 80%以上。处理后的细胞线粒体膜电位没有受到影响。用荧光成像技术评估溶酶体的完整性，结果表明，用葡聚糖包覆的 C70 处理的 L929 细胞没有潜在的毒性。用罗丹明鬼笔环肽染色后观察到，肌动蛋白丝显示出完整的细胞器。DAPI 染色也显示出细胞核在暴露于葡聚糖包覆的 C70 后保持完整。钙黄绿素 AM/PI 流式细胞术分析进一步证实，葡聚糖包覆的 C70 使处理细胞的细胞活力保持在 80%以上，所有浓度均如此。这些结果表明了葡聚糖包覆的 C70 在各种医疗保健应用中的应用前景。
