Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, Brazil.
Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera, Instituto Oswaldo Cruz, Brazil.
J Med Entomol. 2020 Jan 9;57(1):92-103. doi: 10.1093/jme/tjz166.
Males of cryptic or closely related species present great morphological variation in their genitalia, whereas females, such as those of the Chagasi Series of the Psychodopygus Mangabeira, 1941 genus, are more similar. Therefore, our aim was to study the fine structure of the male genitalia of five species of the Chagasi Series to better understand the variation in their morphology and its influence on the copulatory process. The sand fly species were captured in the following Brazilian states: Psychodopygus chagasi (Costa Lima, 1941) (Rondônia), Psychodopygus complexus (Mangabeira, 1941) (Tocantins), Psychodopygus squamiventris maripaensis (Floch & Abonnenc, 1946) (Amapá), Psychodopygus squamiventris squamiventris (Lutz & Neiva, 1912) (Amazonas), and Psychodopygus wellcomei Fraiha, Shaw & Lainson, 1971 (Pará and Ceará). Insects were stored in ethanol 70% (then dehydrated) and dry after they were sputtered with gold. The samples were observed under a scanning electron microscope. Microtrichiae, two types of trichoid sensilla, coeloconic and chaetic sensillae, were observed on the antenna of all species, with no difference between them. Only on the anepimeron of P. squamiventris squamiventris a modified 'racket'-like scale was observed. As for the male genitalia, the setae and structures of each species were fully described, such as the small setae on the paramere apex of the P. squamiventris subspecies, and the grooves present in this region and on the paramere lobe of P. complexus and P. wellcomei, which are impossible to observe with optic microscopy. New information is thus provided on the male genitalia, which can contribute to future bionomic studies of these species.
雄性隐种或近缘种的生殖器在形态上存在很大的变异，而雌性，如 Psychodopygus Mangabeira 属的 Chagasi 系列，更为相似。因此，我们的目的是研究五种 Chagasi 系列沙蝇的雄性生殖器的精细结构，以更好地了解它们形态的变异及其对交配过程的影响。这些沙蝇物种是在以下巴西州捕获的：Psychodopygus chagasi (Costa Lima, 1941)（朗多尼亚州）、Psychodopygus complexus (Mangabeira, 1941)（托坎廷斯州）、Psychodopygus squamiventris maripaensis (Floch & Abonnenc, 1946)（阿马帕州）、Psychodopygus squamiventris squamiventris (Lutz & Neiva, 1912)（亚马逊州）和 Psychodopygus wellcomei Fraiha, Shaw & Lainson, 1971（帕拉州和塞阿拉州）。昆虫被储存在 70%乙醇中（然后干燥），然后用金溅射干燥。样本在扫描电子显微镜下观察。在所有物种的触角上观察到微刚毛、两种毛状感器（栉状感器和刺状感器）、腔锥形感器和刺形感器，它们之间没有区别。只有在 P. squamiventris squamiventris 的后侧片上观察到一种改良的“球拍”状鳞片。至于雄性生殖器，充分描述了每个物种的刚毛和结构，例如 P. squamiventris 亚种的端突上的小刚毛，以及 P. complexus 和 P. wellcomei 中该区域和端突叶上的凹槽，这些在光学显微镜下是无法观察到的。因此，提供了关于雄性生殖器的新信息，这有助于未来对这些物种的生物生态学研究。
