LAMP 产生的 H 离子诱导二聚体 i -motif 作为信号转导子，用于超灵敏电化学检测 DNA。
LAMP-generated H ions-induced dimer i-motif as signal transducer for ultrasensitive electrochemical detection of DNA.
机构信息
Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.
出版信息
Chem Commun (Camb). 2019 Oct 15;55(83):12463-12466. doi: 10.1039/c9cc06738h.
Herein, an ultrasensitive electrochemical biosensor is proposed for the quantification of the Flu A virus biomarker DNA (fDNA), and is based on loop-mediated isothermal amplification-generated hydrogen ions (LAMP-H+) which induce the formation of the dimer i-motif structure (DiMS) for signal transduction, coupled with exonuclease III (ExoIII)-assisted DNA walking for signal dual-amplification.
本文提出了一种超灵敏电化学生物传感器，用于定量检测流感 A 病毒生物标志物 DNA(fDNA)，该传感器基于环介导等温扩增产生的氢离子（LAMP-H+）诱导二聚体 i -motif 结构（DiMS）形成用于信号转导，结合外切酶 III(ExoIII)辅助 DNA 行走进行信号双重扩增。
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