HPLC-DAD-ESI-QTOF-MS/MS 分析骆驼蓬根提取物的成分，并评估其对溴氰菊酯诱导的大鼠心肌损伤的心脏保护作用，通过抑制 NF-κB 信号通路相关氧化应激诱导的炎症和细胞凋亡。
HPLC-DAD-ESI-QTOF-MS/MS profiling of Zygophyllum album roots extract and assessment of its cardioprotective effect against deltamethrin-induced myocardial injuries in rat, by suppression of oxidative stress-related inflammation and apoptosis via NF-κB signaling pathway.
机构信息
Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, 2112, Gafsa, Tunisia.
Laboratoire des Sciences de l'Environnement, Biologie et Physiologie des Organismes Aquatiques, LR18ES41, Faculté des Sciences de Tunis, Université Tunis EL Manar, 2092 Tunis, Tunisia.
出版信息
J Ethnopharmacol. 2020 Jan 30;247:112266. doi: 10.1016/j.jep.2019.112266. Epub 2019 Sep 30.
ETHNOPHARMACOLOGICAL RELEVANCE
Zygophyllum album is widely used to treat many cardiovascular diseases (CVDs) and as anti-inflammatory plant.
AIM OF THE STUDY
This study aimed to investigate the mechanism of the potential protective effects of Zygophyllum album roots extract (ZARE) against myocardial damage and fibrosis induced by a chronic exposure to deltamethrin (DLM) in rats.
MATERIALS AND METHODS
Bioactive compounds present in ZARE were analyzed by HPLC-DAD-ESI-QTOF-MS/MS. In vivo, DLM (4 mg/kg body weight), ZARE (400 mg/kg body weight) and DLM with ZARE were administered to rats orally for 60 days. Biochemical markers (LDH, ALT, CK, CK-MB and cTn-I) were assessed in the plasma by an auto-analyzer. Pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) were evaluated by a sandwich ELISA. NF-κB was quantified at mRNA levels by real time PCR. Heart tissue was used to determine cardiac oxidative stress markers (MDA, PC, SOD, CAT, and GPx). Masson's Trichrome (MT) and Sirius Red (SR) stainings were used for explored fibrosis statues.
RESULTS
Phytochemical analysis using HPLC-DAD-ESI-QTOF-MS/MS revealed the presence of twenty six molecules including phenolic compounds and saponins. ZARE significantly improved the heart injury markers (LDH, ALT, CK, CK-MB and cTn-I), lipid peroxidation (MDA), protein oxidation (PC), antioxidant capacity (SOD, CAT, and GPx), and DNA structure, which were altered by DLM exposure. Moreover, ZARE cotreatment reduced the expressions of NF-κB, decreased plasmatic pro-inflammatory cytokines concentration (TNF-α, IL-1β and IL-6), and suppressed the myocardial collagen deposition, as observed by Sirius Red and Masson's Trichrome staining.
CONCLUSION
ZARE ameliorated the severity of DLM-induced myocardial injuries through improving the oxidative status and reducing profibrotic cytokines production. The ZARE actions could be mediated by downregulation of NF-κB mRNA.
民族药理学相关性
骆驼蓬被广泛用于治疗多种心血管疾病（CVDs）和作为抗炎植物。
研究目的
本研究旨在探讨骆驼蓬根提取物（ZARE）对慢性暴露于溴氰菊酯（DLM）的大鼠心肌损伤和纤维化的潜在保护作用的机制。
材料和方法
通过 HPLC-DAD-ESI-QTOF-MS/MS 分析 ZARE 中的生物活性化合物。在体内，DLM（4mg/kg 体重）、ZARE（400mg/kg 体重）和 DLM 与 ZARE 口服给予大鼠 60 天。通过自动分析仪评估血浆中的生化标志物（LDH、ALT、CK、CK-MB 和 cTn-I）。通过夹心 ELISA 评估促炎细胞因子（TNF-α、IL-1β 和 IL-6）。通过实时 PCR 定量 NF-κB 的 mRNA 水平。心脏组织用于测定心脏氧化应激标志物（MDA、PC、SOD、CAT 和 GPx）。使用 Masson's Trichrome（MT）和 Sirius Red（SR）染色法探索纤维化状态。
结果
使用 HPLC-DAD-ESI-QTOF-MS/MS 的植物化学分析显示存在二十六个分子，包括酚类化合物和皂苷。ZARE 显著改善了由 DLM 暴露引起的心脏损伤标志物（LDH、ALT、CK、CK-MB 和 cTn-I）、脂质过氧化（MDA）、蛋白质氧化（PC）、抗氧化能力（SOD、CAT 和 GPx）和 DNA 结构。此外，ZARE 联合治疗降低了 NF-κB 的表达，降低了血浆促炎细胞因子浓度（TNF-α、IL-1β 和 IL-6），并抑制了心肌胶原沉积，如 Sirius Red 和 Masson's Trichrome 染色观察到的。
结论
ZARE 通过改善氧化状态和减少促纤维化细胞因子的产生，改善了 DLM 诱导的心肌损伤的严重程度。ZARE 的作用可能是通过下调 NF-κB mRNA 介导的。
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