Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Virology. 2019 Dec;538:71-85. doi: 10.1016/j.virol.2019.09.013. Epub 2019 Sep 27.
In order to confirm the existence of W protein in Avian avulavirus 1 (AAvV-1) infected cells, two monoclonal antibodies were prepared. The presence of W protein in cells infected with lentogenic genotype II strain La Sota or velogenic genotype VII strain SG10 was confirmed with immunofluorescence and western blotting assays. W localized to the cytoplasm, whereas W localized to the nucleus. The influence of W protein was investigated in vitro and in vivo with two AAvV-1 strains defective in the W C-terminus. The growth kinetic curves and pathogenicity tests in 3-week-old SPF chickens both showed that the replication abilities of strains with C-terminally deleted W proteins were lower than that of the parental strain. Restoring the appropriate dose of W protein increased the viral titers of these strains. The expression validation and functional exploration of W protein will facilitate our understanding of pathogenic mechanism of AAvV-1.
为了确认禽呼肠孤病毒 1 型（AAvV-1）感染细胞中 W 蛋白的存在，制备了两种单克隆抗体。用免疫荧光和 Western blot 检测证实，弱毒株基因型 II 株 La Sota 或强毒株基因型 VII 株 SG10 感染的细胞中存在 W 蛋白。W 蛋白定位于细胞质，而 W 蛋白定位于细胞核。用两种末端缺失 W 蛋白的 AAvV-1 株在体外和体内研究了 W 蛋白的影响。在 3 周龄 SPF 鸡中的生长动力学曲线和致病性试验均表明，末端缺失 W 蛋白的菌株的复制能力低于亲本株。恢复适当剂量的 W 蛋白增加了这些菌株的病毒滴度。W 蛋白的表达验证和功能探索将有助于我们理解 AAvV-1 的致病机制。
