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使用固相萃取和二维高效液相色谱法纯化和测定植物激素生长素和脱落酸。

Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography.

作者信息

Dobrev P I, Havlícek L, Vagner M, Malbeck J, Kamínek M

机构信息

Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, 16502 Praha 6, Czech Republic.

出版信息

J Chromatogr A. 2005 May 20;1075(1-2):159-66. doi: 10.1016/j.chroma.2005.02.091.

DOI:10.1016/j.chroma.2005.02.091
PMID:15974129
Abstract

A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in "heart cutting" mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors.

摘要

开发了一种基于混合模式反相阴离子交换固相萃取和二维高效液相色谱法分离纯化植物激素生长素和脱落酸的方法。“中心切割”模式的二维高效液相色谱法在纯化这两种激素方面非常有效。其纯化能力足够高，即使使用非选择性检测器也能可靠地在线定量这两种激素。

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