从公共卫生中感兴趣的革兰氏阳性细菌中分离和可视化质粒。

Isolation and Visualization of Plasmids from Gram-Positive Bacteria of Interest in Public Health.

机构信息

Laboratory of Microbiology, Biological Sciences Department, Faculty of Pharmacy, UCIBIO/REQUIMTE, University of Porto, Porto, Portugal.

Department of Microbiology, Ramón y Cajal Research Institute (IRYCIS), Madrid, Spain.

出版信息

Methods Mol Biol. 2020;2075:21-38. doi: 10.1007/978-1-4939-9877-7_2.

DOI:10.1007/978-1-4939-9877-7_2

PMID:31584152

Abstract

This chapter describes the methods to extract and characterize plasmids of Gram-positive bacterial species of interest in public health (biomedicine, veterinary, and food safety) as Staphylococcus, Streptococcus, Enterococcus, Listeria, and Clostridium and lactic acid bacteria. References for detailed plasmid classification are given in order to provide a comprehensive landscape in the interpretation of their plasmidomes.

摘要

本章介绍了从公共卫生（生物医药、兽医和食品安全）中感兴趣的革兰氏阳性细菌物种（葡萄球菌、链球菌、肠球菌、李斯特菌和梭菌以及乳酸菌）中提取和表征质粒的方法。为了全面了解质粒组的解析，给出了详细的质粒分类参考文献。