从乳酸菌中提取质粒DNA的简易方法。
Simple method for extracting plasmid DNA from lactic acid bacteria.
作者信息
Frère J
机构信息
IRBA, Laboratoire de Génétique Microbienne, Université de Caen, France.
出版信息
Lett Appl Microbiol. 1994 Apr;18(4):227-9. doi: 10.1111/j.1472-765x.1994.tb00853.x.
Rapid screening and large-scale plasmid DNA isolation procedures are described for lactic acid bacteria, using glass beads to break cells. The rapid screening procedure allows one to obtain plasmid DNA pellets in less than 1 h. This method has been successfully tested on various bacteria from the genera Lactococcus, Leuconostoc, Lactobacillus, Pediococcus, Streptococcus, Enterococcus and Propionibacterium. This procedure yields plasmid DNA with minor chromosomal and plasmid DNA-degraded form contaminations.
本文描述了一种利用玻璃珠破碎细胞的方法，用于乳酸菌的快速筛选和大规模质粒DNA提取。快速筛选程序可使在不到1小时内获得质粒DNA沉淀。该方法已在乳酸球菌属、明串珠菌属、乳杆菌属、片球菌属、链球菌属、肠球菌属和丙酸杆菌属的多种细菌上成功测试。该程序产生的质粒DNA中染色体和质粒DNA降解形式的污染较少。
Zotero 插件