IATE, Univ Montpellier, CIRAD, INRA, Montpellier SupAgro, Montpellier, France.
Food Funct. 2019 Oct 16;10(10):6882-6891. doi: 10.1039/c9fo01528k.
Phytoprostanes (PhytoPs) and phytofurans (PhytoFs) are isoprostanoids that result from the peroxidation of α-linolenic acid and are biomarkers of oxidative stress in plants and humans. These compounds exhibit several interesting biological activities (e.g. neuroprotection and anti-inflammatory activities). The aim of this research was to add value to coffee pulp (CP), cocoa husk (CH) and cocoa pod husk (CPH) by identifying and quantifying PhytoPs and PhytoFs by liquid chromatography-tandem mass spectrometry. The contents of PhytoPs and PhytoFs in CP, CH, and CPH were, respectively, 654.6, 474.3 and 179.9, and 543.2, 278.0 and 393.8 ng per g dry weight (dw). The main PhytoP found in CP (171.37 ng per g dw) and CPH (37.12 ng per g dw) was 9-epi-9-F1t-PhytoP, while ent-9-L1t-PhytoP was the most abundant in CH (109.78 ng per g dw). The main PhytoF found in all sources was ent-16(RS)-13-epi-ST-Δ14-9-PhytoF, at 196.56, 126.22, and 207.57 ng per g dw in CP, CH, and CPH, respectively. We provide the first complete profile of PhytoPs and PhytoFs for these agro-residues, which could be used in the functional food industry for enriching food or as nutritional supplements.
植物前列腺素（PhytoPs）和植物呋喃（PhytoFs）是α-亚麻酸过氧化的异前列腺素，是植物和人类氧化应激的生物标志物。这些化合物表现出多种有趣的生物活性（例如神经保护和抗炎活性）。本研究旨在通过液相色谱-串联质谱法鉴定和定量咖啡果皮（CP）、可可壳（CH）和可可荚壳（CPH）中的 PhytoPs 和 PhytoFs，从而为 CP、CH 和 CPH 增加附加值。CP、CH 和 CPH 中 PhytoPs 和 PhytoFs 的含量分别为 654.6、474.3 和 179.9，以及 543.2、278.0 和 393.8ng/g 干重（dw）。CP（37.12ng/g dw）和 CPH（37.12ng/g dw）中主要的 PhytoP 是 9-epi-9-F1t-PhytoP，而 CH（109.78ng/g dw）中含量最丰富的是 ent-9-L1t-PhytoP。所有来源中主要的 PhytoF 是 ent-16(RS)-13-epi-ST-Δ14-9-PhytoF，分别为 CP、CH 和 CPH 中的 196.56、126.22 和 207.57ng/g dw。我们首次为这些农业残留物提供了 PhytoPs 和 PhytoFs 的完整图谱，可用于功能性食品工业，以丰富食品或作为营养补充剂。
