Huang Dezhi, Ying Haojun, Jiang Dongneng, Liu Fei, Tian Ye, Du Chunlan, Zhang Liqun, Pu Xiaoyun
Department of Clinical Laboratory, Xinqiao Hospital, Army Medical University, Chongqing, 400037, China.
Department of Clinical Laboratory, Xinqiao Hospital, Army Medical University, Chongqing, 400037, China.
Anal Biochem. 2020 Jan 1;588:113468. doi: 10.1016/j.ab.2019.113468. Epub 2019 Oct 1.
Interleukin 6 (IL-6) is an interleukin that acts as both a proinflammatory and anti-inflammatory cytokine. It can be used as a potential diagnostic biomarker for sepsis. The aim of this study was to establish an easy-to-use detection kit for rapid, quantitative and on-site detection of IL-6. To develop the new IL-6 quantitative detecting kit, a double-antibody sandwich immunofluorescent assay was employed based on europium nanoparticles (Eu-np) combined with lateral flow immunoassay (LFIA). The performance of the new developed kit was evaluated in the aspects of parallel analysis, linearity, sensitivity, precision, accuracy, specificity and clinical sample analysis. Two-hundred and fourteen serum samples were used to carry out the clinical sample analysis. The new IL-6 quantitative detecting kit exhibited a wide linear range (2-500 pg/mL) and a good sensitivity (0.37 pg/mL). The intra-assay coefficient of variation (CV) and the inter-assay CV were 5.92%-8.87% and 7.59%-9.04%, respectively. The recovery rates ranged from 102% to 106%. Furthermore, a high correlation (n = 214, r = 0.9756, p < 0.01) was obtained when compared with SIEMENS CLIA IL-6 kit. Thus, the new quantitative method for detecting IL-6 has been successfully established. The results indicated that the newly-developed strip based on Eu-np combined with LFIA was a facile, fast, highly sensitive, low-cost, reliable biosensor and suitable for rapid and point-of-care test (POCT) for IL-6 in serum.
白细胞介素6（IL-6）是一种兼具促炎和抗炎作用的细胞因子。它可作为脓毒症潜在的诊断生物标志物。本研究旨在建立一种易于使用的检测试剂盒，用于快速、定量和现场检测IL-6。为开发新型IL-6定量检测试剂盒，采用了基于铕纳米颗粒（Eu-np）结合侧向流免疫分析（LFIA）的双抗体夹心免疫荧光分析方法。从平行分析、线性、灵敏度、精密度、准确度、特异性和临床样本分析等方面对新开发试剂盒的性能进行了评估。使用214份血清样本进行临床样本分析。新型IL-6定量检测试剂盒显示出较宽的线性范围（2 - 500 pg/mL）和良好的灵敏度（0.37 pg/mL）。批内变异系数（CV）和批间CV分别为5.92% - 8.87%和7.59% - 9.04%。回收率在102%至106%之间。此外，与西门子CLIA IL-6试剂盒相比，获得了高度相关性（n = 214，r = 0.9756，p < 0.01）。因此，成功建立了检测IL-6的新定量方法。结果表明，新开发的基于Eu-np结合LFIA的试纸条是一种简便、快速、高灵敏度、低成本、可靠的生物传感器，适用于血清中IL-6的快速即时检测（POCT）。
