Delfraissy J F, Wallon C, Boué F, Vazquez A, Galanaud P
Eur J Immunol. 1985 May;15(5):433-8. doi: 10.1002/eji.1830150504.
The monoclonal antibody OKT3 can induce T suppressor cells of the in vitro antibody response when used at concentrations as low as 10 ng/ml and in cultures containing as few as 4% OKT3-pretreated T cells. These suppressor cells are radioresistant, cyclosporin sensitive and belong to the T4 as well as the T8 subpopulations. In contrast with the mitogenic effect of OKT3 the suppressor activity can be induced and expressed in highly monocyte-depleted cultures. Moreover F(ab')2 fragments of OKT3, although not mitogenic, are able to induce suppressor cells. Thus cross-linking of the T3 molecule (such as that realized when intact OKT3 IgG and monocytes are present) is not required to induce suppressor cells among resting normal T cells.
单克隆抗体OKT3在浓度低至10纳克/毫升且培养物中仅含4%经OKT3预处理的T细胞时，即可在体外诱导抗体反应的T抑制细胞。这些抑制细胞具有放射抗性，对环孢菌素敏感，属于T4和T8亚群。与OKT3的促有丝分裂作用相反，抑制活性可在高度单核细胞耗竭的培养物中诱导并表达。此外，OKT3的F(ab')2片段虽无促有丝分裂作用，但能够诱导抑制细胞。因此，在静止的正常T细胞中诱导抑制细胞并不需要T3分子的交联（如完整的OKT3 IgG和单核细胞存在时所实现的那样）。
