Romero P A, Friedlander P, Fellows L, Evans S V, Herscovics A
FEBS Lett. 1985 May 20;184(2):197-201. doi: 10.1016/0014-5793(85)80606-2.
The effects of manno-1-deoxynojirimycin (ManDJN) and 2,5-dihydroxymethyl-3,4-dihydroxypyrrolidine (DMDP) were compared in IEC-6 intestinal epithelial cells in culture. ManDJN caused complete inhibition of N-linked complex oligosaccharide synthesis whereas a maximum of 80% inhibition was obtained with DMDP. HPLC showed similar endo H-sensitive oligosaccharides for control and treated cells. ManDJN caused a large increase in the levels of labeled Man7-9 GlcNAc and a decrease in Man5GlcNAc. DMDP produced similar changes except that the increase in Man7-9GlcNAc was less pronounced and some increase in glucosylated oligosaccharides was observed. Since the major oligosaccharides found in DMDP-treated cells were non-glucosylated, its primary effect on complex oligosaccharide synthesis is not due to inhibition of glucosidases, in contrast to what has been reported for influenza virus-infected MDCK cells [(1984) J. Biol. Chem. 259, 12409-12413].
在培养的IEC-6肠上皮细胞中比较了甘露糖-1-脱氧野尻霉素（ManDJN）和2,5-二羟甲基-3,4-二羟基吡咯烷（DMDP）的作用。ManDJN导致N-连接复合寡糖合成完全抑制，而DMDP最多可抑制80%。高效液相色谱显示对照细胞和处理细胞的内切糖苷酶H敏感寡糖相似。ManDJN导致标记的Man7-9 GlcNAc水平大幅增加，而Man5GlcNAc减少。DMDP产生了类似的变化，只是Man7-9GlcNAc的增加不太明显，并且观察到糖基化寡糖有一些增加。由于在DMDP处理的细胞中发现的主要寡糖是非糖基化的，与流感病毒感染的MDCK细胞的报道情况相反[(1984)《生物化学杂志》259, 12409 - 12413]，其对复合寡糖合成的主要作用不是由于对糖苷酶的抑制。
