Analytical Chemistry - Center for Electrochemical Sciences (CES), Faculty of Chemistry and Biochemistry, Ruhr University Bochum, Universitätsstr. 150, D-44780, Bochum, Germany; Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Professor Lineu Prestes, 748 05513-970, São Paulo, Brazil.
Analytical Chemistry - Center for Electrochemical Sciences (CES), Faculty of Chemistry and Biochemistry, Ruhr University Bochum, Universitätsstr. 150, D-44780, Bochum, Germany.
Anal Chim Acta. 2019 Dec 9;1087:36-43. doi: 10.1016/j.aca.2019.08.049. Epub 2019 Aug 27.
The Scanning Bipolar Electrochemical Microscope (SBECM) allows precise positioning of an electrochemical micro-probe serving as bipolar electrode that can be wirelessly interrogated by coupling the electrochemical detection reaction with an electrochemiluminescent reporting process. As a result, the spatially heterogeneous concentrations of an analyte of interest can be converted in real time into a map of sample reactivity. However, this can only be achieved upon optimization of the analytical performance ensuring adequate sensitivity. Here, we present the evaluation and optimized operation of the SBECM for the detection of small changes in local O concentrations. Parameters for achieving an improved sensitivity as well as possibilities for improving the signal-to-noise ratio in the optical signal readout are evaluated. The capability of the SBECM for O detection is shown at controlled conditions by recording the topography of a patterned sample and monitoring O evolution from a photoelectrocatalyst material.
扫描双极电化学显微镜（SBECM）允许精确定位作为双极电极的电化学微探针，通过将电化学检测反应与电化学发光报告过程相结合，可以对其进行无线询问。结果，感兴趣的分析物的空间不均匀浓度可以实时转换为样品反应性的图谱。然而，只有在优化分析性能以确保足够的灵敏度的情况下才能实现这一点。在这里，我们展示了 SBECM 在检测局部 O 浓度小变化方面的评估和优化操作。评估了实现灵敏度提高的参数以及改进光学信号读出中信噪比的可能性。通过记录图案化样品的形貌并监测光电催化剂材料中 O 的演化，在受控条件下展示了 SBECM 对 O 检测的能力。
