Ino Kosuke, Mockaitis Tomas, Shikuwa Ryota, Oba Kimiharu, Hiramoto Kaoru, Morkvenaite-Vilkonciene Inga, Abe Hiroya, Shiku Hitoshi
Graduate School of Engineering, Tohoku University, Sendai, 980-8579, Japan.
Laboratory of Bioelectrochemical Technologies, Department of Nanotechnology, State Research Institute Centre for Physical Sciences and Technology, 10257, Vilnius, Lithuania.
Anal Sci. 2025 May;41(5):557-569. doi: 10.1007/s44211-025-00723-x. Epub 2025 Feb 7.
Electrochemiluminescence (ECL) is a chemiluminescence phenomenon triggered by electrochemical reactions and is widely used for (bio)chemical analyses and electrochemical bioimaging. Compared to fluorescence sensing, ECL sensing reduces background noise by eliminating autofluorescence associated with excitation light. In addition, compared with conventional electrochemical imaging with scanning electrochemical microscopes, ECL imaging is faster as it requires no scanning. Furthermore, unlike electrode arrays, ECL devices can function without complex wiring, simplifying their construction. These characteristics render ECL sensing a useful analytical tool. Recently, ECL sensing has been widely used for in vitro cell analysis due to high demand for biochips in regenerative medicine, drug screening, and microphysiological systems. This review focuses on recent advancements in ECL-based cell analysis with applications for the detection of HO, respiration activity, cell adhesion, lipid membranes, and bipolar electrochemistry-based devices.
电化学发光（ECL）是一种由电化学反应引发的化学发光现象，广泛应用于（生物）化学分析和电化学生物成像。与荧光传感相比，ECL传感通过消除与激发光相关的自发荧光来降低背景噪声。此外，与使用扫描电化学显微镜的传统电化学成像相比，ECL成像速度更快，因为它无需扫描。而且，与电极阵列不同，ECL装置无需复杂布线即可工作，简化了其构造。这些特性使ECL传感成为一种有用的分析工具。近年来，由于再生医学、药物筛选和微生理系统对生物芯片的需求很高，ECL传感已广泛用于体外细胞分析。本综述重点介绍基于ECL的细胞分析的最新进展及其在检测HO、呼吸活性、细胞粘附、脂质膜和基于双极电化学的装置方面的应用。
