Chapeau C, Gutkowska J, Schiller P W, Milne R W, Thibault G, Garcia R, Genest J, Cantin M
J Histochem Cytochem. 1985 Jun;33(6):541-50. doi: 10.1177/33.6.3158698.
The localization of two synthetic fragments of the C-terminal portion of atrial natriuretic factor: Arg 101-Tyr 126 which displays full biological activity and Leu 94-Arg 109 which is completely devoid of biological activity, has been investigated by immunohisto- and immunocytochemical methods in the heart of mammals (rat, mouse, guinea pig, hamster, rabbit, cat, dog, man) and nonmammalian vertebrates toad (Bufo marinus), frog (Rana catesbeiana), fish (Cyprinus carpio, Puntius schwanenfeldi, Cichlosoma biocellatum, Carrasius auratus), snake (Python reticulatus) and hen. Antibodies against the synthetic fragments of ANF were raised in rabbits and used either for immunofluorescence (Coons' technique), immunohistochemistry (unlabeled antibody technique) or immunocytochemistry (protein A-gold technique). Results obtained by immunofluorescence and by the unlabeled antibody technique were similar: antibodies against Arg 101-Tyr 126 ANF allowed visualization of granulated cardiocytes in the atria of all mammals. While the reaction was very strong in rat and mouse, it was less so in the rabbit and very weak in all other species studied including man. Antibodies against Leu 94-Arg 109 ANF produced a reaction only in the rat and mouse. In nonmammalian vertebrates, the reaction was always much stronger in atria than ventricles of all species with both antibodies.
利用免疫组织化学和免疫细胞化学方法，对心房钠尿肽C端两个合成片段进行了定位研究。这两个片段分别是具有完整生物活性的精氨酸101 - 酪氨酸126片段，以及完全没有生物活性的亮氨酸94 - 精氨酸109片段。研究对象包括哺乳动物（大鼠、小鼠、豚鼠、仓鼠、兔子、猫、狗、人）和非哺乳动物脊椎动物蟾蜍（海蟾蜍）、青蛙（牛蛙）、鱼类（鲤鱼、施氏魮、双斑丽体鱼、鲫鱼）、蛇（网纹蟒）和母鸡。针对心房钠尿肽合成片段的抗体在兔子体内产生，用于免疫荧光（孔斯技术）、免疫组织化学（未标记抗体技术）或免疫细胞化学（蛋白A - 金技术）。免疫荧光和未标记抗体技术得到的结果相似：抗精氨酸101 - 酪氨酸126心房钠尿肽的抗体能使所有哺乳动物心房中的颗粒心肌细胞可视化。在大鼠和小鼠中反应非常强烈，在兔子中反应较弱，在包括人类在内的所有其他研究物种中反应非常微弱。抗亮氨酸94 - 精氨酸109心房钠尿肽的抗体仅在大鼠和小鼠中产生反应。在非哺乳动物脊椎动物中，使用这两种抗体时，所有物种心房中的反应总是比心室中的反应强烈得多。
