一种针对大鼠心房利钠因子（ANF）的鼠单克隆抗体，它与小鼠ANF发生交叉反应。

A murine monoclonal antibody against rat atrial natriuretic factor (ANF) which cross-reacts with mouse ANF.

作者信息

Milne R, Gutkowska J, Thibault G, Schiller P, Charbonneau C, Genest J, Cantin M

出版信息

Mol Immunol. 1987 Feb;24(2):127-32. doi: 10.1016/0161-5890(87)90084-8.

Abstract

A monoclonal antibody (MAb), 2H2, against rat synthetic atrial natriuretic factor (ANF) (Arg101-Tyr126) recognizes native ANF related peptides. The lack of reactivity of 2H2 with amino-terminal truncated ANF peptides implicates the two amino terminal arginine residues of ANF in the 2H2 epitope. Similarly, poor immunoreactivity of human ANF indicates the participation of isoleucine 110. Arginines 101 and 102 and isoleucine 110 may thus participate in a conformational epitope recognized by 2H2 or alternatively, substitution for, or elimination of these residues may alter the conformation of the 2H2 epitope. The MAb shows little cross-reactivity with extracts of rabbit atria but recognizes ANF related peptides in mouse and hamster atrial extracts. 2H2 also identifies immunoreactive ANF in histological sections of rat, mouse and hamster atria.

摘要

一种针对大鼠合成心钠素（ANF）（精氨酸101 - 酪氨酸126）的单克隆抗体（MAb）2H2可识别天然ANF相关肽。2H2与氨基末端截短的ANF肽缺乏反应性，这表明ANF的两个氨基末端精氨酸残基参与了2H2表位。同样，人ANF的免疫反应性较差表明异亮氨酸110的参与。因此，精氨酸101和102以及异亮氨酸110可能参与了由2H2识别的构象表位，或者，这些残基的取代或消除可能会改变2H2表位的构象。该单克隆抗体与兔心房提取物几乎没有交叉反应，但可识别小鼠和仓鼠心房提取物中的ANF相关肽。2H2还可在大鼠、小鼠和仓鼠心房的组织切片中识别有免疫反应性的ANF。