Department of Ecology, Evolution, and Marine Biology, University of California, Santa Barbara, CA 93106-9620, USA.
Philos Trans R Soc Lond B Biol Sci. 2019 Nov 25;374(1786):20190096. doi: 10.1098/rstb.2019.0096. Epub 2019 Oct 7.
Single-cell genomics has transformed many fields of biology, marine microbiology included. Here, we consider the impact of single-cell genomics on a specific group of marine microbes-the planktonic marine archaea. Despite single-cell enabled discoveries of novel metabolic function in the marine thaumarchaea, population-level investigations are hindered by an overall lower than expected recovery of thaumarchaea in single-cell studies. Metagenome-assembled genomes have so far been a more useful method for accessing genome-resolved insights into the Marine Group II euryarchaea. Future progress in the application of single-cell genomics to archaeal biology in the ocean would benefit from more targeted sorting approaches, and a more systematic investigation of potential biases against archaea in single-cell workflows including cell lysis, genome amplification and genome screening. This article is part of a discussion meeting issue 'Single cell ecology'.
单细胞基因组学已经改变了许多生物学领域，包括海洋微生物学。在这里，我们考虑了单细胞基因组学对海洋微生物特定群体的影响 - 浮游海洋古菌。尽管单细胞技术揭示了海洋泉古菌的新代谢功能，但在单细胞研究中，古菌的总体回收率低于预期，这阻碍了种群水平的调查。迄今为止，宏基因组组装基因组是一种更有用的方法，可以深入了解海洋 II 组广古菌。单细胞基因组学在海洋古菌生物学中的应用的未来进展将受益于更有针对性的分类方法，以及更系统地研究单细胞工作流程中对古菌的潜在偏见，包括细胞裂解、基因组扩增和基因组筛选。本文是“单细胞生态学”讨论会议的一部分。
