Brotcorne-Lannoye A, Maenhaut-Michel G, Radman M
Mol Gen Genet. 1985;199(1):64-9. doi: 10.1007/BF00327511.
It has been proposed that the mutation fixation processes stimulated by SOS induction result from an induced infidelity of DNA replication (Radman 1974). The aim of this study was to determine if mutator mutations in the E. coli DNA polymerase III might affect UV-induced mutagenesis. Using a phage lambda mutation assay which can discriminate between targeted and untargeted mutations, we show that the polC74 mutator mutation (Sevastopoulos and Glaser 1977) primarily affects untargeted mutagenesis, which occurs in a recA1 genetic background and is amplified in the recA+ genetic background. The polC74 mutation also increases the UV-induced mutagenesis of the bacterial chromosome. These results suggest that DNA polymerase III is involved in the process of UV-induced mutagenesis in E. coli.
有人提出，SOS诱导所刺激的突变固定过程源于DNA复制诱导的错误倾向（拉德曼，1974年）。本研究的目的是确定大肠杆菌DNA聚合酶III中的诱变突变是否会影响紫外线诱导的诱变作用。使用一种能够区分靶向突变和非靶向突变的噬菌体λ突变检测方法，我们发现polC74诱变突变（塞瓦斯托普洛斯和格拉泽，1977年）主要影响非靶向诱变，这种诱变发生在recA1遗传背景中，并在recA+遗传背景中被放大。polC74突变还增加了紫外线诱导的细菌染色体诱变作用。这些结果表明，DNA聚合酶III参与了大肠杆菌中紫外线诱导的诱变过程。
