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[包含致癌基因和逆转录病毒基因类似物的人类基因组区域。II. 一类新的逆转录病毒样元件]

[Regions of human genome-containing analogs of oncogenes and retroviral genes. II. A new class of retrovirus-like elements].

作者信息

Chumakov I M, Zabarovskiĭ E R, Prasolov V S, Mett V L, Kiselev L L

出版信息

Mol Biol (Mosk). 1985 Mar-Apr;19(2):425-32.

PMID:3158802
Abstract

Earlier we have found that recombinant phage lambda gp5 contains the sequences homologous to v-mos oncogene and retroviruses. After the nucleotide sequence determination we have found the region with homology to U5 part of retroviral LTR. Adjacent to this region are sequences complementary to 3'-end of tRNAMet. Numerous transcripts reacting with subcloned U5 probe from gp5 are present in polyadenylated RNA fraction from human cells. The humane genome contains several copies of these region, with two of them residing in gp5 locus. On the basis of these data we deduced the presence in the human genome of a new class of retroviral-like elements, existing probably as part of new human endogeneous retrovirus (HuEV).

摘要

早些时候我们发现,重组噬菌体λ gp5含有与v-mos癌基因和逆转录病毒同源的序列。在确定核苷酸序列后,我们发现了与逆转录病毒长末端重复序列(LTR)的U5部分同源的区域。与该区域相邻的是与tRNA^Met 3'末端互补的序列。在来自人类细胞的聚腺苷酸化RNA组分中存在许多与从gp5亚克隆的U5探针反应的转录本。人类基因组包含这些区域的几个拷贝,其中两个位于gp5基因座中。基于这些数据,我们推断人类基因组中存在一类新的类逆转录病毒元件,可能作为新的人类内源性逆转录病毒(HuEV)的一部分存在。

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1
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Mol Biol (Mosk). 1985 Mar-Apr;19(2):425-32.
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